Experience efficient and effortless application with the NuAction™ Dye Extraction Kit which uses gel-filtration technology for the removal of dye terminators from sequencing reactions, ensuring high-quality sequencing results.
NuAction™ Dye Extraction Kit
Price range: $261.00 through $3,173.00
Purchase this item and get Product Description
The NuAction™ Dye Extraction Kit offers an efficient solution for removing dye terminators from sequencing reactions, ensuring high-quality sequencing results. Whether using the spin column or 96-well plate format, this kit employs gel-filtration technology to remove unincorporated terminators quickly and effectively. The procedure is streamlined, requiring only two short centrifugation steps, making it ideal for laboratories requiring fast turnaround times. The pre-hydrated gel-filtration material eliminates the need for extensive preparation, providing convenience and reliability in the sequencing workflow. With the NuAction™ Dye Extraction Kit, researchers can achieve superior sequencing results compared to traditional methods like ethanol precipitation. Dye blobs, which often hinder sequence readability, are effectively eliminated, resulting in clear and accurate sequencing data. Additionally, the high signal intensities obtained with this kit enable long read lengths, enhancing the depth and quality of sequencing analysis. The principle behind the Dye Extraction procedure lies in the gel-filtration material, which selectively retains dye terminators while allowing labeled DNA fragments to pass through. This principle ensures the efficient removal of contaminants without compromising the integrity of the sequencing samples. The versatility of the NuAction™ Dye Extraction Kit extends to its compatibility with various sequencing platforms, including ABI PRISM and MegaBACE sequencers, making it suitable for a wide range of sequencing applications. Overall, the NuAction™ Dye Extraction Kit represents a valuable tool for researchers seeking a fast, reliable, and efficient method for sequencing reaction cleanup, ensuring high-quality sequencing data for downstream analysis and interpretation.
Kit Contents
● Dye Extraction 96 Well Plates (4)
● Collection Plates, 48-Well (4)
Technical Parameters
● Binding Capacity: 10–20 µl
● Elution Volume: 10–20 µl
● Compatible Sequencers: ABI PRISM 377, 373, 310, 3100, or 3700,
MegaBACE 1000, or CEQ 2000
● Format: Tube (Dye Extraction 2.0 Spin Kit), 96-well plate (Dye
Extraction 96 Kit)
● Processing: Manual
● Removal of <10mers to 17–40mers dye terminator proteins
Applications
● Removal of dye terminators from sequencing reactions
● Suitable for ABI PRISM and MegaBACE sequencers
● Ideal for sequencing reactions requiring high-quality data and long
read lengths
Product FAQs
What is the principle behind the NuAction™ Dye Extraction Kit?
The NuAction™ Dye Extraction Kit utilizes gel-filtration technology to selectively retain dye terminators while allowing labeled DNA fragments to pass through, ensuring efficient removal of contaminants.
How does the NuAction™ Dye Extraction Kit compare to ethanol precipitation?
The NuAction™ Dye Extraction Kit offers superior sequencing results compared to ethanol precipitation, eliminating dye blobs and ensuring clear and accurate sequencing data.
Can the NuAction™ Dye Extraction Kit be used with different sequencing platforms?
Yes, the NuAction™ Dye Extraction Kit is compatible with various sequencing platforms, including ABI PRISM and MegaBACE sequencers.
What is the processing time for the NuAction™ Dye Extraction Kit?
The processing time for the NuAction™ Dye Extraction Kit is minimal, requiring only 10-18 minutes depending on the format used, making it ideal for laboratories requiring fast turnaround times.
| Quantity | Plus Kit: 250 preps, Plus Kit: 50 preps, Standard Kit: 24 X 96 preps, Standard Kit: 4 X 96 preps |
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