The miRibo™ Kits offer efficient microRNA (miRNA) and total RNA purification, even from small samples, enabling high-quality RNA suitable for various molecular biology applications.
miRibo™ Kits
$506.00 – $2,450.00
Purchase this item and get Product Description
miRibo™ Kits provide a robust solution for the purification of total RNA, including miRNA, from a wide range of animal tissues and cells. With protocols designed for effective RNA extraction from difficult-to-lyse tissues and small samples, these kits offer flexibility and reliability for diverse research needs. The kits are available in micro, mini, and 96-well plate formats, catering to different sample volumes and throughput requirements. One of the notable features of miRibo™ Kits is their ability to efficiently enrich miRNA and RNAs smaller than 200 nucleotides, allowing for sensitive downstream applications. The kits enable the purification of both total RNA and an miRNA-enriched fraction separately, providing flexibility for different experimental setups. The purified RNA is of high purity, making it suitable for applications such as PCR, real-time RT-PCR, microarray analysis, and RNA-seq.
The principle behind miRibo™ Kits involves phenol/guanidine-based lysis of samples combined with silica membrane-based purification of total RNA. Amzol™ Lysis Reagent, included in the kits, facilitates sample lysis, inhibits RNases, and removes cellular DNA and proteins from the lysate. The innovative AutEx™ system allows for automated miRNA purification, streamlining the workflow and ensuring reproducible results.
Kit Contents
● NuAction™ MinElute Spin Columns
● Collection Tubes (1.5 ml and 2 ml)
● Amzol™ Lysis Reagent
● RNase-Free Reagents and Buffers
Technical Parameters
● Applications: PCR, qPCR, real-time RT-PCR, microarray
● Elution volume: 14-30 µl
● Purification of: Total RNA, miRNA, poly A+ mRNA, DNA or protein (miRNA, total RNA)
● Sample amount: Up to 4 sections, each with a thickness up to 10 µm and a surface area up to 250 mm^2 (automated protocol on AutEx™: up to 2 sections)
● Processing: Manual (protocol for automated processing on AutEx™ available)
● Main sample type: FFPE tissue samples
● Format: Spin column
● Technology: Silica technology
● Yield: Varies
Applications
● PCR
● qPCR
● Real-time RT-PCR
● Microarray analysis
● RNA-seq
Product FAQs
What types of samples are compatible with miRibo™ Kits?
miRibo™ Kits are compatible with a wide range of animal tissues and cells, including difficult-to-lyse tissues and small samples.
Can miRibo™ Kits be used for miRNA enrichment?
Yes, miRibo™ Kits efficiently enrich miRNA and RNAs smaller than 200 nucleotides, providing flexibility for different experimental setups.
Is automation possible with miRibo™ Kits?
Yes, miRibo™ Kits can be automated on the AutEx™ system, allowing for high-throughput processing and reproducible results.
What downstream applications are supported by RNA purified with miRibo™ Kits?
RNA purified with miRibo™ Kits is suitable for various downstream applications, including PCR, real-time RT-PCR, microarray analysis, and RNA-seq.
| Quantity | 96 well kits: 4X96preps, Micro: 50 preps, Mini: 50 preps |
|---|
Related products
-
miRibo™ Sera/Plasma Kit
$634.00The miRibo™ Sera/Plasma Kit is an advanced solution designed for the precise and efficient extraction of miRNA from serum and plasma samples. This kit ensures the high-quality isolation of miRNA, which is essential for accurate downstream analysis such as qRT-PCR, biomarker discovery, and translational research, making it a powerful tool for molecular biology labs.Select options This product has multiple variants. The options may be chosen on the product page Show more -
miRibo™ FFPE Kit
$596.00The miRibo™ FFPE Kit offers a streamlined solution for efficient purification of total RNA, including miRNA, from formalin-fixed, paraffin-embedded (FFPE) tissue sections, enabling reliable molecular biology applications.Select options This product has multiple variants. The options may be chosen on the product page Show more -
miRibo™ Ultra AutEx™ Kit
$432.00 – $4,437.00Effortlessly isolate high-quality total RNA, including miRNA and other small RNA, from serum and plasma samples with the miRibo™ Ultra AutEx™ Kit, offering reliable automated processing for medium-to-high-throughput applications.Select options This product has multiple variants. The options may be chosen on the product page Show more -
miRibo™ LNA Custom Probe PCR Assay
$319.00 – $363.00Elevate your miRNA research with the miRibo™ LNA Custom Probe PCR Assay. Designed for exquisite sensitivity and specificity, this customizable assay empowers researchers to precisely quantify miRNA expression levels, even from ultra-low input RNA samples. With its streamlined workflow and advanced LNA-enhanced technology, this assay delivers reliable results in just 2 hours, making it an indispensable tool for miRNA biomarker discovery, pathway analysis, and more.Select options This product has multiple variants. The options may be chosen on the product page Show more
Updating…