Anti·His Antibodies, BSA-free

Price range: $943.00 through $1,127.00

Highly specific mouse monoclonal antibody for detecting His-tagged proteins in molecular biology applications.

Purchase this item and get 94-112 Points
Purchase this item and get 94-112 Points
Product Description

The Anti-His Antibodies, BSA-free is a powerful tool designed for the rapid and efficient purification of viral nucleic acids from plasma and serum samples. This kit is meticulously crafted for molecular biology applications, offering highly specific detection of C-terminal, N-terminal, and internal His tags. With its superior sensitivity and specificity in detecting Anti-His epitopes and HHHHH epitopes, researchers can confidently rely on this kit for a wide range of research endeavors.

Featuring monoclonal mouse Anti His Antibodies, including the RGS-His Antibody, BSA-free, Tetra-His Antibody, BSA-free, and Penta-His Antibody. BSA-free, this kit enables the detection of recombinant proteins carrying His tags with exceptional precision. Each variant targets a distinct His tag epitope, providing researchers with flexibility and versatility in their experimental designs. Moreover, these antibodies are prepared under serum-free conditions, ensuring the absence of viruses, mycoplasma, and contaminating immunoglobulins, thereby maintaining the integrity of the samples.

The performance of the Anti-His Antibodies, BSA-free is exemplified by its ability to detect numerous proteins via their His tags. Furthermore, the negligible cross-reactivity with crude E. coli, yeast, mammalian, or insect cell lysates ensures the specificity of the detection process.

The versatility of the Anti-His Antibodies, BSA-free extends to its applications, including colony, dot, and western blotting procedures, screening for positive expression clones, monitoring expression levels and stability of His-tagged proteins, as well as immunoprecipitation and ELISA. Additionally, the kit is suitable for immunocytochemistry and immunohistochemistry, making it an indispensable tool for various research settings.

 

Kit Contents

● 100 µg lyophilized mouse anti-His antibody
● For preparing a 1000 mL working solution

Technical Parameters​

● Form: Lyophilized
● Isotype: Mouse IgG1
● Cross-reactivity: Negligible with crude E. coli, yeast, mammalian, or insect cell lysates
● Working solution: 1000 mL

Applications

● Colony, dot, and western blotting procedures
● Screening for positive expression clones
● Monitoring expression levels and stability of His-tagged proteins
● Immunoprecipitation and ELISA
● Immunocytochemistry and immunohistochemistry

Product FAQs
What is the sensitivity of the Anti-His Antibody in dot blot assays?

The sensitivity of the Anti-His Antibody in dot blots is 0.5 ng, ensuring reliable detection of His-tagged proteins even at low concentrations.

Can this antibody detect both N-terminal and C-terminal His tags?

Yes, the Anti-His Antibody specifically detects N-terminal His tags containing the epitope RGSHHHH, making it suitable for detecting proteins with both N-terminal and C-terminal His tags.

Is it necessary to perform any additional purification steps before using this antibody?

No, this antibody is prepared under serum-free conditions, ensuring high purity and activity. It does not require additional purification steps before use in various assays.

How long does it take to prepare the working solution from the lyophilized antibody?

The lyophilized antibody can be reconstituted to prepare a 1000 mL working solution, which can typically be done within a few minutes, allowing for quick and efficient use in experiments.

Quantity

Penta His: 100µg, RGS His: 100µg, Tetra His: 100µg

SKU: PA8201 Category:
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