Anti·His HRP Conjugate Kit

Anti·His HRP Conjugate Kit

$596.00 – $675.00

High-sensitivity HRP-conjugated antibodies for direct detection of His-tagged proteins in various molecular biology assays.

Purchase this item and get 59-67 Points
Purchase this item and get 59-67 Points
Product Description

The Anti-His HRP Conjugate Kit offers a convenient solution for sensitive detection of recombinant proteins carrying His tags without the need for secondary antibodies. This kit includes Penta His HRP Conjugate, a stabilized stock solution of HRP-conjugated anti-His antibodies, along with blocking reagents and buffers. The antibodies in this kit specifically bind to N-terminal, C-terminal, and internal His tags, enabling the direct detection of His-tagged proteins in western blots, dot blots, colony blots, ELISA, and other immunoassay procedures. With its high sensitivity and speed, this kit is ideal for researchers working with His-tagged proteins in molecular biology applications.

Kit Contents

● Sensitivity in dot blots: 10 pg
● Sensitivity in western blots: 50 pg
● Antibody isotype: Mouse IgG1
● Cross-reactivity: Negligible with crude E. coli, yeast, mammalian, or
insect cell lysates
● Form: Stabilized stock solution
● Working solution: 125–250 mL

Technical Parameters​

● Western-, dot-, and colony-blotting procedures
● ELISA and other immunoassay procedures

Applications

● Genomic studies
● Cancer research
● Complex sample analysis
● High-throughput sequencing projects

Product FAQs
What is the sensitivity of the Anti-His HRP Conjugate Kit in dot blot assays?

The sensitivity of the kit in dot blots is 10 pg, ensuring reliable detection of His-tagged proteins even at low concentrations.

Do I need to use a secondary antibody with this kit for detection?

No, the Anti-His HRP Conjugate Kit eliminates the need for secondary antibodies, saving time and simplifying the immunodetection procedure.

Can I use this kit for both N-terminal and C-terminal His tags?

Yes, the antibodies in the kit are capable of detecting N-terminal, C-terminal, and internal His tags, providing flexibility in detecting different His-tagged proteins.

How should I store the kit components for long-term use?

The components of the kit, including the blocking reagent, blocking reagent buffer, and Penta His HRP Conjugate, should be stored according to the instructions provided in the manual to maintain their stability and effectiveness.

Quantity

Penta His HRP: 125µL, RGS His HRP: 125µL
SKU: PA1479 Category:
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