Achieve fast and specific quantification of RNA targets with the OptiZip™ SYBR® Green RT-PCR Kit, designed for real-time one-step RT-PCR using SYBR® Green detection.
OptiZip™ SYBR® Green RT-PCR Kits
$4,660.00
Purchase this item and get Product Description
The OptiZip™ SYBR® Green RT-PCR Kit revolutionizes RNA quantification, offering rapid and accurate results in real-time one-step RT-PCR assays. Powered by AvBlend™ technology and an optimized master mix, this kit slashes run times by up to 60%, catering to both standard and fast cyclers. By combining a hot start mechanism with a novel qRT-PCR buffer system, it ensures high specificity and sensitivity, even for low-copy RNA targets. Additionally, an optimized RT mix facilitates cDNA synthesis in just 10 minutes. The kit’s convenience extends further with the option for storage at 2–8°C, ensuring flexibility and ease of use.
The OptiZip™ SYBR® Green RT-PCR Kit delivers highly specific and sensitive results over a wide dynamic range on both standard and fast cyclers. The fluorescent dye SYBR® Green in the master mix enables the analysis of many different targets without having to synthesize target-specific labeled probes. A specially developed fast RT-PCR buffer contains the novel PCR additive AvBlend™, which significantly reduces denaturation, annealing, and extension times. A balanced combination of K+ and NH4+ ions in the PCR buffer promotes specific primer annealing, enabling high RT-PCR specificity and sensitivity. In addition, FusionTaq™ Pro DNA Polymerase requires only 5 minutes at 95°C for activation and provides a stringent hot start, preventing the formation of nonspecific products. The optimized OptiZip™ RT Mix enables cDNA synthesis in just 10 minutes.
Kit Contents
For 2000 reactions:
FusionTaq™ Pro DNA Polymerase (3 X 1.7 mL) OptiZip™ SYBR® Green RT-PCR Buffer (2 X 1.7 mL) AvBlend™ additive (3 X 1 mL)
SYBR® Green dye (3 X 0.075 mL)
ROX dye (3 X 0.075 mL)
OptiZip™ RT Mix (3 X 0.015 mL)
dNTP mix (dATP, dCTP, dGTP, dTTP) (3 X 0.2 μ=mL)
Nuclease-Free Water (2 X 1 mL)
Technical Parameters
● FusionTaq™ Pro DNA Polymerase Activation: 5 min at 95°C
● Detection Method: SYBR® Green
● Buffer Composition: Balanced combination of NH4+ and K+ ions
● Fluorescent Dyes: SYBR® Green
● Reverse Transcription Time: 10 min
● Storage: -20°C for long-term storage, 2–8°C for short-term storage
● Shelf Life: 12 months
Applications
The OptiZip™ SYBR® Green RT-PCR Kit is suitable for the following applications:
● Real-time quantification of RNA targets
● Multiplex real-time quantification of RNA targets in a single tube
● Suitable for various real-time cyclers, including Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche, and Agilent instruments
Product FAQs
How does the OptiZip™ SYBR® Green RT-PCR Kit achieve such rapid results?
The kit leverages advanced AvBlend™ technology and an optimized master mix to expedite the RT-PCR process, reducing run times significantly.
Can I store the master mix at room temperature?
For optimal performance, we recommend storing the master mix at 2–8°C. However, it can be kept at room temperature for short periods during experiments.
Is this kit suitable for both standard and fast cyclers?
Yes, the OptiZip™ SYBR® Green RT-PCR Kit is compatible with both standard and fast cyclers, offering flexibility in experimental setups.
How long does the reverse transcription step take?
The reverse transcription step with the OptiZip™ RT Mix takes only 10 minutes, enabling rapid cDNA synthesis for efficient RNA quantification.
| Reactions | 2000 |
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