The OptiSense™ PCR Kits offer unparalleled specificity, sensitivity, and speed in real-time PCR. These kits feature a novel hot-start mechanism for enhanced specificity and accurate quantification of targets. With built-in visual indicators, they ensure precise reaction setup and process safety. The kits enable multiplex capability, detecting up to 5 targets in a single tube, optimizing workflow efficiency. Offering stability of up to 100 hours at 30°C, they provide flexibility in experimental setups.
OptiSense™ PCR Kits
Price range: $146.00 through $4,709.00
Purchase this item and get Product Description
OptiSense™ PCR Kits offer enhanced specificity, sensitivity, and process safety, setting new standards in real-time PCR. The novel hot-start mechanism significantly increases specificity, while optimized chemistry produces accurate quantitative results from cDNA or gDNA. Built-in visual indicators ensure correct pipetting, enhancing process safety.
1. OptiSense™ SYBR Green PCR Kit (SYBR qPCR kit):
● Sets new standards in SYBR Green-based qPCR with enhanced specificity, sensitivity, and speed.
● Utilizes a novel hot-start mechanism for increased PCR specificity and accurate quantification of targets.
● Features a built-in visual indicator for correct pipetting, enhancing process safety.
● Allows accurate quantification over a wide dynamic range on any real-time PCR cycler.
● Provides ROX for precise quantification on various real-time PCR cyclers.
2. OptiSense™ Probe PCR Kit (probe qPCR kit):
● Enables accurate probe-based real-time PCR analysis of cDNA or gDNA targets.
● Employs an antibody-mediated hot-start mechanism for enhanced specificity and efficiency.
● Ensures stability of reactions for up to 100 hours at room temperature, ideal for high-throughput setups.
● Enables sensitive detection of even low target amounts, ensuring reliable qPCR results.
● Includes an in-built visual tracking system for accurate pipetting and process safety.
3. OptiSense™ Multiplex PCR Kits (multiplex qPCR kits):
● Designed for fast and reliable quantification of up to 5 cDNA or gDNA targets in a single tube.
● Utilizes Q-bond technology and an optimized master mix for ultrafast multiplex real-time PCR.
● Ensures highly sensitive qPCR on any real-time cycler without the need for optimization.
● Features an in-built visual tracking system to aid accurate pipetting and prevent human errors.
● Allows inclusion of internal control RNA for process monitoring when combined with the OptiSense™ Reverse Transcription Kit.
Kit Contents
● OptiSense™ SYBR Green PCR Kit or OptiSense™ Probe PCR Kit or OptiSense™ Multiplex PCR Kit (depending on variant) MasterMix
● Template DNA or cDNA (not included)
● Primer-probe sets (for Probe PCR Kit and Multiplex PCR Kit)
● ROX passive reference dye (separate tube, optional)
● OptiSense™ Template Dilution Buffer
Technical Parameters
● Reaction stability: Up to 100 hours at 30°C
● Detection limit: Down to one copy
● Multiplexing: Up to 5 targets in 1 tube (Multiplex PCR Kit)
● Storage: Master mix can be stored at 2–8°C for up to 12 months
Applications
The OptiSense™ PCR Kits isapplicable for the following requirements:
● Gene expression analysis
● Diagnostic assays
● Epidemiological studies
● Suitable for basic research, clinical diagnostics, and high-throughput applications
Product FAQs
What is the shelf life of OptiSense™ PCR Kits?
OptiSense™ PCR Kits offer a shelf life of up to 12 months when stored at 2–8°C, ensuring long-term reliability for your experiments.
Can OptiSense™ PCR Kits detect single-copy targets?
Yes, the OptiSense™ PCR Kits are designed to detect targets down to a single copy, providing unparalleled sensitivity for your assays.
Are OptiSense™ PCR Kits compatible with all real-time PCR cyclers?
Yes, OptiSense™ PCR Kits are compatible with a wide range of real-time PCR cyclers, ensuring flexibility and ease of use in your laboratory setup.
Do OptiSense™ PCR Kits require optimization for different targets?
No, OptiSense™ PCR Kits feature optimized chemistry that eliminates the need for time-consuming optimization, allowing you to obtain reliable results quickly and consistently.
Can OptiSense™ PCR Kits be used for high-throughput applications?
Yes, the OptiSense™ PCR Kits are suitable for high-throughput applications, offering stability of up to 100 hours at 30°C and enabling efficient processing of large sample batches with confidence.
| Detection type | Probe, Multiplex Probe, SYBR Green |
|---|---|
| Reactions | 100, 500, 2500, 7500 |
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