The miRibo™ Ultra Sera/Plasma Kit offers a cutting-edge solution for researchers requiring reliable isolation of total RNA, including miRNA, from serum and plasma samples. This advanced kit incorporates a phenol-free protocol, eliminating the need for phase separation, and is compatible with automation systems like the AutEx™ platform for efficient RNA extraction.
miRibo™ Ultra Sera/Plasma Kit
$506.00 – $2,450.00
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The miRibo™ Ultra Sera/Plasma Kit is a cutting-edge solution designed for the isolation of total RNA, including miRNA, from serum and plasma samples. With a minimum input volume of 200 μl, this kit provides researchers with a reliable method to extract high-quality RNA without the need for laborious phase separation steps.
Featuring a phenol-free protocol, the miRibo™ Ultra Sera/Plasma Kit offers the convenience of benchtop processing while ensuring consistent and pure RNA for downstream applications. Utilizing MinElute spin column technology, this kit enables the isolation of RNA in small elution volumes of approximately 20 μl, ideal for applications requiring minimal sample input.
The performance of the miRibo™ Ultra Sera/Plasma Kit rivals that of its predecessor, the miRNeasy Serum/Plasma Kit, delivering comparable RNA yields and quality. This kit excels in efficiently isolating total RNA, including miRNA, from serum and plasma samples, enabling comprehensive analysis of extracellular vesicle RNA content.
The principle behind the miRibo™ Ultra Sera/Plasma Kit lies in its innovative spin-column technology. By leveraging Buffer RPL and Buffer RPP, this kit ensures efficient lysis of extracellular vesicles and precipitation of contaminants such as plasma proteins. The subsequent binding of RNA to the silica membrane and removal of contaminants through wash steps culminate in the elution of highly pure RNA suitable for downstream applications.
The user-friendly procedure of the miRibo™ Ultra Sera/Plasma Kit involves a simple mixing of serum or plasma with lysis buffers, followed by centrifugation and RNA binding to the MinElute spin column. After thorough washing to remove impurities, the elution of total RNA (>18 nucleotides) is achieved using RNase-free water.
Applications of the miRibo™ Ultra Sera/Plasma Kit span a wide range of molecular biology studies, including biomarker discovery, expression profiling, next-generation sequencing, quantitative real-time RT-PCR, and microarray analysis. Its versatility and reliability make it indispensable for researchers exploring the complexities of cell-free and exosomal RNA from plasma or serum samples.
In summary, the miRibo™ Ultra Sera/Plasma Kit represents a significant advancement in RNA isolation technology, offering researchers a powerful tool to unlock the potential of extracellular RNA in elucidating disease mechanisms, identifying biomarkers, and advancing personalized medicine.
Kit Contents
● Lysis Buffer
● Precipitation buffer
● miRibo™ Spin Columns
● Nuclease-free Water
● Ultra-clean Columns
Technical Parameters
● Sample Input: Minimum of 200 μl of serum or plasma
● Elution Volume: Approximately 20 μl
● RNA Size: >18 nucleotides
● Automation: Compatible with automation systems like AutEx™ platform
Applications
● Biomarker discovery
● Expression profiling
● Next-generation sequencing
● Quantitative real-time RT-PCR
● Microarray analysis
Product FAQs
Can this kit be used for both serum and plasma samples?
Yes, the miRibo™ Ultra Sera/Plasma Kit is suitable for isolating total RNA from both serum and plasma samples.
What is the minimum input volume required for sample processing?
The kit requires a minimum input volume of 200 μl of serum or plasma for RNA extraction.
Is automation possible with this kit?
Yes, the kit is compatible with automation systems like the AutEx™ platform for streamlined RNA extraction.
Does the kit provide consistent RNA yields and quality?
Yes, the kit offers similar RNA yields and quality compared to previous kits, ensuring reliable performance for downstream applications.
| Kit type | Micro, Mini, 96 well kits |
|---|---|
| Preparations | 50 preps, 4 x 96 preps |
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