CrystalGene™ Accessories enable the purification of high-molecular-weight DNA from various sample types, ensuring long-term stability and suitability for sensitive downstream applications.
CrystalGene™ Accessories
Price range: $103.00 through $684.00
Purchase this item and get Product Description
CrystalGene™ Accessories are designed to work seamlessly with Puregene Kits to purify high-molecular-weight DNA from a wide range of sample types. These accessories ensure the extraction of DNA that is highly stable, making it ideal for long-term storage and archiving. The purification process involves cell lysis using an anionic detergent in the presence of a DNA stabilizer, followed by RNA removal with RNase A, and protein removal via salt precipitation. The genomic DNA is then precipitated with alcohol and dissolved in a hydration solution. The resulting DNA typically exhibits an A260/A280 ratio between 1.7 and 1.9 and sizes up to 200 kb. This high-quality DNA is suitable for applications such as PCR, SNP analysis, Southern blotting, NGS, and long-read sequencing. The accessories include essential reagents like RBC Lysis Solution, Cell Lysis Solution, Protein Precipitation Solution, and DNA Hydration Solution. These components ensure efficient and reproducible DNA purification, catering to the needs of both research and clinical laboratories. With the in-house manufacturing solution, the CrystalGene™ Accessories maintain consistent quality and performance, supporting reliable and accurate results in various genetic and genomic studies.
Kit Contents
● RBC Lysis Solution
● Cell Lysis Solution
● Protein Precipitation Solution
● DNA Hydration Solution
● Proteinase K
● RNase A
● Glycogen
Technical Parameters
● DNA yield: High-molecular-weight DNA (100–200 kb)
● Purity: A260/A280 ratio between 1.7 and 1.9
● Storage: DNA can be stored at 2–8°C, –20°C, or –80°C
● Scalability: Suitable for large-volume samples
Applications
● RBC Lysis Solution
● Cell Lysis Solution
● Protein Precipitation Solution
● DNA Hydration Solution
● Proteinase K
● RNase A
Product FAQs
What is the main use of CrystalGene™Accessories?
They are used for purifying high-molecular-weight DNA suitable for long-term storage and sensitive downstream applications.
What does the DNA purification process involve?
It includes cell lysis, RNA removal, protein precipitation, and DNA precipitation and hydration.
What type of DNA does the Puregene method yield?
It yields high-quality DNA with sizes up to 200 kb and a purity ratio (A260/A280) between 1.7 and 1.9.
Can the purified DNA be used for next-generation sequencing?
Yes, the purified DNA is suitable for NGS, PCR, SNP analysis, Southern blotting, and long-read sequencing.
| Product | Enzyme, Solution |
|---|---|
| Type | RBC Lysis Solution, Cell Lysis Solution, Protein Precipitation Solution, DNA Hydration Solution, Glycogen, Proteinase K, RNase A |
| Quantity | 5mL, 50mL, 100mL, 125mL, 450mL, 500mL, 1000mL, 500μL, 650μL |
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