Avam® Reverse Transcriptase is specifically engineered to synthesize a specific complementary DNA strand from RNA or ssDNA.
Avam® Reverse Transcriptase
Price range: $127.00 through $1,097.00
Purchase this item and get Product Description
The Avam® Reverse Transcriptase included in this kit is a modified, recombinant form derived from the Moloney Murine Leukemia Virus (M-MuLV), purified from Escherichia coli. This enzyme is specifically designed to synthesize a complementary DNA (cDNA) strand from either RNA or single-stranded DNA (ssDNA) templates in the presence of a primer. Notable features of the Avam® Reverse Transcriptase include its high thermal stability, making it suitable for use at higher temperatures (optimal activity at 50°C), and its ability to generate full-length cDNA strands, even from complex RNA templates. Additionally, it exhibits increased sensitivity in reverse transcription quantitative PCR (RT-qPCR) and reverse transcription PCR (RT-PCR) assays.
The enzyme is accompanied by a 10x RT Reaction Buffer containing Tris-HCl (pH 8.3), KCl, MgCl2, and DTT, providing optimal conditions for efficient cDNA synthesis.
Each unit of the Avam® Reverse Transcriptase is defined as the amount required to incorporate 1 nanomole of dTTP into an acid-insoluble material in 10 minutes at 37°C, using poly(A) x oligo(dT)12-18 as a template primer. In terms of performance, the enzyme exhibits high purity (>90%) and is free from DNase and RNase contamination, ensuring reliable results in downstream applications.
The Avam® Reverse Transcriptase is particularly beneficial for synthesizing full-length cDNA templates for RT-qPCR and two-step RT-PCR assays, as well as for cDNA synthesis in molecular cloning and cDNA library construction. It serves as a crucial tool in RNA analysis, enabling researchers
to study gene expression and RNA function with high accuracy and efficiency.
Kit Contents
200 U/μL Avam® Reverse Transcriptase (1 X 0.05 μL)
10X RT Reaction Buffer (1 X 0.1 mL)
Technical Parameters
● Storage: -20°C without a defrost cycle
● Reverse Transcriptase Concentration: 200 U/μL
● RT Reaction Buffer Composition: 500 Tris-HCl (pH 8.3), 750 mM KCl, 30 mM MgCl2, 100 mM DTT
Applications
● Full-length cDNA template synthesis for RT-qPCR and two-step RT-PCR assays
● cDNA synthesis for molecular cloning
● cDNA library construction
● RNA analysis
Product FAQs
What is the storage condition for the kit components?
All components should be stored at -20°C without a defrost cycle.
What is the specific activity of the Avam® Reverse Transcriptase enzyme?
The Avam® Reverse Transcriptase enzyme has a specific activity of 200 U/μL.
Can the kit be used for RT-qPCR assays?
Yes, the kit is suitable for synthesizing cDNA for RT-qPCR assays, offering increased sensitivity and high efficiency.
Is the enzyme RNase-free?
Yes, the enzyme is free from RNase contamination, ensuring the integrity of RNA templates during cDNA synthesis.
How should the enzyme be inactivated after use?
Enzyme inactivation should be carried out at 85°C for 5 minutes to ensure complete inactivation before downstream applications.
| Quantity | 10,000U, 100,000U, 50,000U |
|---|
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