Evolve your RNA quantification experiments with the OptiZip™ Multiplex RT-PCR Kit, designed to streamline multiplex, real-time one-step RT-PCR assays, allowing for the precise detection of up to 4 RNA targets in a single tube while offering accelerated results with time savings of up to 50%.
OptiZip™ Multiplex RT-PCR Kits
Price range: $1,202.00 through $5,590.00
Purchase this item and get Product Description
Experience unparalleled efficiency and accuracy in RNA quantification with the OptiZip™ Multiplex RT-PCR Kit. This advanced kit revolutionizes the quantification process by enabling simultaneous detection of multiple RNA targets in a single well, eliminating the need for cumbersome optimization steps. By incorporating cutting-edge AVFuse technology and an optimized master mix, this kit ensures rapid and reliable quantification across both standard and fast cyclers, delivering consistent and reproducible results with minimal effort.
The OptiZip™ Multiplex RT-PCR Kit is not only about speed; it’s also about precision. With its ability to discriminate small differences in target amount and detect reference genes alongside up to 3 targets in the same tube, this kit empowers researchers to delve deeper into complex gene expression studies with confidence. Additionally, the convenience of storing the master mix at 2–8°C adds flexibility to experimental setups, allowing for seamless integration into your laboratory workflow.
Kit Contents
● 2X OptiZip™ Multiplex RT-PCR MasterMix (with or without ROX dye) (3 X 1.7 mL)
● OptiZip RT Mix (1 X 0.1 mL)
● Nuclease-Free Water
Technical Parameters
● FusionTaq™ Pro DNA Polymerase Activation: 5 min at 95oC
● Detection Method: SYBR Green
● Buffer Composition: Balanced combination of NH4+ and K+ ions
● Fluorescent Dyes: SYBR Green
● Reverse Transcription Time: 20 min
● Storage: -20°C for long-term storage, 2–8°C for short-term storage
● Shelf Life: 12 months
Applications
The OptiZip™ Multiplex RT-PCR Kit is suitable for the following applications:
● Gene expression analysis of RNA targets
● Molecular diagnostics and pathogen detection
● Viral load monitoring and biomarker discovery
● Environmental and agricultural research
● Pharmaceutical and biotechnology applications
Product FAQs
How does the OptiZip™ Multiplex RT-PCR Kit achieve such rapid results?
Leveraging advanced AVFuse technology and an optimized master mix, this kit significantly reduces RT-PCR run times, enabling faster results.
Can I store the master mix at room temperature?
For optimal performance, we recommend storing the master mix at 2–8°C. However, it remains stable at room temperature for short periods during experiments.
Is this kit suitable for both standard and fast cyclers?
Yes, the OptiZip™ Multiplex RT-PCR Kit is compatible with both standard and fast cyclers, offering versatility in experimental setups.
What is the shelf life of this kit?
The kit has a shelf life of 12 months when stored properly at the recommended temperature.
| Reference Dye | With ROX, Without ROX |
|---|---|
| Reactions | 400 reactions, 2000 reactions |
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