Accelerate real-time RT-PCR with OptiScript™ Cell RT-PCR Kits, ideal for rapid high-throughput gene expression analysis directly from cultured cells without RNA purification.
OptiScript™ Cell RT-PCR Kits (200)
Price range: $2,051.00 through $2,458.00
Purchase this item and get Product Description
The OptiScript™ Cell RT-PCR Kits revolutionizes the process of real-time RT-PCR, offering a streamlined and efficient solution for rapid gene expression analysis. Specifically designed for experiments requiring swift high-throughput analysis directly from cultured cells, this kit provides a comprehensive set of buffers optimized for the rapid preparation of cell lysates.
Traditionally, the process of RNA extraction from cultured cells followed by subsequent purification can be time-consuming and labor-intensive. However, with the OptiScript™ Cell RT-PCR Kits, this step is entirely eliminated, significantly reducing the overall experimental timeline. Moreover, the kit’s buffers not only facilitate cell lysis but also stabilize cellular RNA while simultaneously eliminating genomic DNA, ensuring the integrity and purity of the extracted RNA.
Utilizing OptiScript™ lysates directly in real-time one-step RT-PCR and multiplex, real-time one-step RT-PCR further enhances the efficiency and speed of gene expression analysis. The kit supports various detection formats, including singleplex detection with SYBR Green or sequence-specific probes, as well as multiplex detection with sequence-specific probes (with or without ROX passive reference dye).
With the OptiScript™ Cell RT-PCR Kits, researchers can bypass the cumbersome RNA purification step, enabling immediate startup using optimized reagents and protocols. This feature is particularly advantageous in high-throughput settings, where rapid analysis of numerous samples is required.
The OptiScript™ Cell RT-PCR Kits is not only characterized by its speed and efficiency but also by its reliability and reproducibility. Results obtained from experiments using this kit demonstrate highly reproducible outcomes, even in high-throughput analysis settings. Additionally, the kit’s innovative design ensures sensitive and specific quantification, with minimal interference from genomic DNA contamination.
Overall, the OptiScript™ Cell RT-PCR Kits represents a cutting-edge solution for researchers seeking to expedite gene expression analysis without compromising on quality or reliability. By streamlining the workflow and eliminating the need for RNA purification, this kit empowers researchers to accelerate their research and make significant strides in understanding gene expression dynamics.
Kit Contents
Probe:
OptiScript™ Cell One-Step Buffer Set
2X OptiScript™ Probe RT-PCR MasterMix
OptiScript™ RT Mix
Multiplex Probe:
OptiScript™ Cell One-Step Buffer Set
2X OptiScript™ Multiplex RT-PCR MasterMix (with or without ROX dye)
OptiScript™ Multiplex RT Mix
SYBR Green:
OptiScript™ Cell One-Step Buffer Set
2X OptiScript™ SYBR Green RT-PCR MasterMix
OptiScript™ RT Mix
Technical Parameters
● Detection Type: Probe
● Multiplex Probe: Available
● SYBR Green: Available
● Quantity: 1 kit
● Storage Conditions: -20°C
● Shelf Life: 12 months
Applications
The OptiScript™ Cell RT-PCR Kits is well-suited for:
● Validation of siRNA-mediated gene knockdown
● Screening potential drugs
● Detection of stimulated gene regulation
Product FAQs
What is the unique feature of the OptiScript™ Cell RT-PCR Kits?
The OptiScript™ Cell RT-PCR Kits allows direct real-time RT-PCR from cultured cells without the need for RNA purification, saving valuable time.
Can I use this kit for multiplex analysis?
Yes, the kit supports multiplex analysis in both 96- and 384-well formats, providing flexibility for various experimental setups.
How long does it take to obtain lysates using this kit?
Lysates can be obtained in just 12 minutes with a simple 3-step procedure, enabling quick processing of samples for downstream analysis.
| Detection type | Probe, Multiplex Probe, SYBR Green |
|---|---|
| Reference Dye | ROX, No ROX, N/A |
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