MaxHis™ Ni-NTA Plates

$335.00

MaxHis™ Ni-NTA Plates offer a robust solution for molecular biology applications, providing researchers with enhanced signal-to-noise ratio and reproducibility in a convenient multiwell-based assay system.

Purchase this item and get 33 Points
Purchase this item and get 33 Points
Product Description

MaxHis™ Ni-NTA Plates offer a robust solution for the immobilization of His-tagged proteins in protein interaction assays, presenting a convenient and efficient platform for various molecular biology applications. Each well of these 96-well plates is coated with Ni-NTA, ensuring uniform and directed immobilization of His-tagged biomolecules. This coating facilitates enhanced signal-to-noise ratio and reproducibility, crucial for reliable assay results.

The solid-phase format of MaxHis™ Ni-NTA Plates streamlines assay development by reducing the need for extensive optimization, thereby accelerating the experimental workflow. Their compatibility with crude lysates further expands their utility, allowing efficient immobilization of His-tagged proteins even in complex biological samples.

Utilizing MaxHis™ Ni-NTA Plates simplifies quantitation of His-tagged molecules, enabling applications such as ELISA, hybridoma and antibody screening, drug discovery, and screening of expression clones and engineered enzymes. In summary, MaxHis™ Ni-NTA Plates provide researchers with a versatile and reliable tool for protein interaction assays, offering enhanced performance, convenience, and reproducibility in a multiwell-based assay system.

Kit Contents

25 mL nickel charged resin

Technical Parameters​

● Epitope detected: Histidine residue epitope
● Format: Plate based
● Instrument: Manual
● Ligand on bead or plate: Ligand on plate
● Tag: 6xHis tag

Applications

● Quantitation of His-tagged molecules
● ELISA
● Hybridoma and antibody screening
● Drug discovery
● Screening of expression clones and engineered enzymes

Product FAQs
What is the storage condition for MaxHis™ Ni-NTA Plates?

MaxHis™ Ni-NTA Plates should be stored at the recommended temperature of 2-8°C until the expiration date. Avoid freezing the plates, as this may affect their performance.

Can MaxHis™ Ni-NTA Plates be used with crude lysates?

Yes, MaxHis™ Ni-NTA Plates are designed for use with crude lysates, allowing efficient immobilization of His-tagged proteins even in complex samples.

What is the binding capacity of MaxHis™ Ni-NTA Plates?

MaxHis™ Ni-NTA Plates offer a high binding capacity for His-tagged proteins, ensuring efficient immobilization and reliable assay results. However, specific binding capacities may vary based on experimental conditions.

Are MaxHis™ Ni-NTA Plates compatible with automated assay systems?

While MaxHis™ Ni-NTA Plates are designed for manual use, they can be adapted for use with automated assay systems with appropriate modifications to accommodate the plate format and handling requirements.

Quantity

5 plates

SKU: CA8761 Category:
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