The ZeroEndo™ Plasmid Kits provide an advanced solution for high-yield purification of endotoxin-free plasmid DNA, essential for sensitive applications like transfection and gene therapy. Integrated endotoxin removal ensures superior purity, surpassing conventional methods. Obtain reliable results with ease, ideal for demanding molecular biology research.
ZeroEndo™ Plasmid Kits
Price range: $459.00 through $999.00
Purchase this item and get Product Description
The ZeroEndo™ Plasmid Kits revolutionize plasmid DNA purification, offering an integrated approach to ensure endotoxin-free DNA of exceptional purity by combining an efficient endotoxin removal step with anion-exchange-based purification, this kit surpasses conventional methods, delivering high-quality DNA suitable for even the most sensitive molecular biology applications. Their streamlined workflow and superior performance make it an indispensable tool for researchers working in fields such as gene therapy, transfection, and gene silencing. With the ZeroEndo™ Plasmid Kits, the purification process is optimized for maximum efficiency and reliability. Bacterial lysates are rapidly cleared using QIAfilter Cartridges, eliminating the need for time-consuming centrifugation steps. The subsequent purification of plasmid DNA ensures high yields and purity, with minimal hands-on time required. The endotoxin removal buffer effectively eliminates endotoxins released during lysis, resulting in DNA with <0.1 EU/µg DNA. This level of purity is crucial for enhancing transfection efficiencies and minimizing unwanted immune responses in downstream applications. The exceptional performance of the ZeroEndo™ Plasmid Kits is underscored by its ability to yield high-quality DNA suitable for a wide range of applications. Whether performing transfections in primary or sensitive cell lines, conducting gene therapy research, or exploring gene silencing mechanisms, researchers can rely on the purified DNA obtained from this kit to deliver consistent and reproducible results. Furthermore, the kit is adaptable to various culture volumes, offering flexibility for both small-scale experiments and large-scale preparations. By addressing the challenge of endotoxin contamination head-on, the ZeroEndo™ Plasmid Kits empower researchers to focus on their scientific objectives with confidence. Its robust performance, ease of use, and compatibility with sensitive applications make it an indispensable tool for laboratories engaged in cutting-edge molecular biology research. With the ZeroEndo™ Plasmid Kits, researchers can achieve superior results and unlock new insights into the complexities of gene expression, regulation, and function.
Kit Contents
● Purification Tips
● Reagents
● Filter Cartridges
● Endotoxin-free Buffers
Technical Parameters
● Culture Volume/Starting Material:
● Maxi Kit: 100–250 mL culture volume
● Mega Kit: 500 mL – 2.5 liters culture volume
● Giga Kit: 2.5 liters culture volume
● Plasmid Type: High-copy, low-copy, cosmid DNA
● Processing: Manual (gravity flow)
● Sample per Run: 1 sample per run
● Time per Run:
Maxi Kit: 150 min
Mega Kit: 220 min
Giga Kit: 310 min
● Yield:
Maxi Kit: <500 µg
Mega Kit: <2.5 mg
Giga Kit: <10 mg
Applications
● Transfection, including primary, sensitive, and suspension cells
● Gene therapy research
● Gene silencing
● Microinjection
Product FAQs
How do the ZeroEndo™ Plasmid Kits ensure endotoxin-free DNA?
The kit integrates an efficient endotoxin removal step into the purification process, yielding plasmid DNA containing <0.1 EU/µg DNA
What is the advantage of using Filter Cartridges in this kit?
Filter Cartridges efficiently clear bacterial lysates, saving time by eliminating the need for centrifugation, resulting in high-quality DNA yields.
Can the purified DNA be used in sensitive transfection experiments?
Yes, the endotoxin-free DNA obtained with this kit is highly suited for reproducible and reliable results in transfection, even in primary and sensitive cell lines.
Are the kits suitable for large-scale plasmid DNA preparations?
Yes, the ZeroEndo™ Plasmid Kits are available in Maxi, Mega, and Giga formats, accommodating various culture volumes and yielding up to 10 mg of purified DNA, making it suitable for both small and large-scale applications.
| Reactions | Buffer Set, Giga Kit: 100 buffers, Maxi Kit: 10 reactions, Mega Kit: 10 reactions |
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