The ViralReady™ RNA Prep & Amp Kit offers an innovative liquid-based approach for rapid sample preparation and sensitive qPCR detection of enveloped RNA viruses from human samples.
ViralReady™ RNA Prep & Amp UM Kit
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The ViralReady™ RNA Prep & Amp UM Kit revolutionizes the process of viral RNA epidemiology with its innovative liquid-based approach. Engineered for ultrafast sample preparation and sensitive real-time PCR detection, this kit is specifically designed for enveloped RNA viruses such as coronaviruses. It streamlines the workflow by seamlessly integrating sample preparation and real-time PCR-based virus detection into a single kit, reducing turnaround time and increasing testing frequency.
Compatible with human samples collected in various non-fixation transport media, including nasal, oro- and nasopharyngeal swabs, as well as neat saliva and gargle samples, the ViralReady™ RNA Prep & Amp UM Kit offers flexibility and versatility in sample types. Its simplified workflow requires only three pipetting steps directly in the PCR reaction vessel, making it ideal for laboratories seeking to enhance efficiency and productivity.
Designed for compatibility with any assay and real-time PCR cycler, this kit features a 4-plex (RT)-PCR capability, allowing for the simultaneous detection of up to 4 targets, including viral targets and/or controls. This multiplexing capability enhances the kit’s utility in epidemiological research, enabling the detection of multiple pathogens or variants of interest in a single run.
Moreover, the ViralReady™ RNA Prep & Amp UM Kit includes two controls—an inhibition control and a sampling control—that ensure reliable interpretation of results. The inhibition control consists of a synthetic RNA with a unique sequence, enabling the monitoring of successful amplification and the detection of instrument or chemistry failures. The sampling control targets human transcripts, confirming the presence of human material in the sample and validating the integrity of RNA materials.
With a two-phase hot-start procedure and stable RT included in the master mix, the kit ensures high specificity and sensitivity in real-time RT-PCR. This robust performance is further enhanced by optimized chemistry, delivering results comparable to standard qPCR workflows.
In summary, the ViralReady™ RNA Prep & Amp UM Kit offers a comprehensive solution for rapid and sensitive viral RNA detection, with an end-to-end liquid-based workflow that simplifies sample preparation and accelerates time to result. It is an indispensable tool for laboratories engaged in epidemiological research, outbreak surveillance, and monitoring of viral variants.
Kit Contents
● ViralReady™ RNA UM Prep Buffer (1 X 0.24 mL)
● 4X ViralReady™ RNA Master Mix, (1 X 0.5 mL)
● RNA IC Template + Assay (1 X 0.2 mL)
● Human Sampling IC Assay (1 X 0.1 mL)
● ROX(1X1mL)
● Nuclease-free water (1 X 1.9 mL)
Technical Parameters
● Time to Result: Under 1 hour
● Sample Type: Human samples collected in non-fixation transport media
● Throughput: Up to 2600 samples per real-time PCR cycler
● Compatibility: Compatible with any assay and real-time PCR cycler
● Multiplexing Capability: 4-plex (RT)-PCR capability for detection of up to 4 targets
Applications
● Rapid detection of enveloped RNA viruses such as coronaviruses
● Epidemiological research purposes
● Monitoring of virus variants of concern
Product FAQs
Is the kit compatible with various sample types?
Yes, the kit is compatible with human samples collected in non-fixation transport media, including nasal, oro- and nasopharyngeal swabs, as well as neat saliva and gargle samples.
Can the kit be automated?
Yes, the kit can be seamlessly integrated into automated workflows with standard lab equipment for any throughput needs.
What controls are included in the kit?
The kit includes two controls: an inhibition control and a sampling control, for reliable interpretation of results.
What is the turnaround time for obtaining results?
Results can be obtained in under 1 hour from start to finish, including incubation and hands-on time.
| Product | Viral RNA UM Kit, Buffer 1 & 2 Kit, SARS-CoV-2 Positive control Kit (2 x 220μL) |
|---|---|
| Reactions | 100, 600, 2400, N/A |
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