VersaKit™ Viral DNA/RNA Kit

VersaKit™ Viral DNA/RNA Kit

$595.00

Experience efficient purification of viral and bacterial total nucleic acids from challenging samples like wastewater, stool, biosolids, and gut material with the VersaKit™ Viral DNA/RNA Kit.

Purchase this item and get 59 Points
Purchase this item and get 59 Points
Product Description

The VersaKit™ Viral DNA/RNA Kit is meticulously designed to overcome the challenges associated with isolating nucleic acids from difficult samples such as wastewater, stool, biosolids, and gut material. Leveraging advanced technology, this kit ensures efficient purification of viral and bacterial total nucleic acids, even in the presence of PCR inhibitors commonly found in these complex matrices.

The key feature of this kit lies in its ability to efficiently remove inhibitory substances using Inhibitor Removal Technology. This innovative approach guarantees the elimination of problematic compounds, such as undigested plant material in gut samples or heme compounds from lysed red blood cells in stool samples, thus resulting in highly pure DNA and RNA suitable for a wide range of downstream applications.

Utilizing a combination of chemical lysis and bead-beating protocols, the VersaKit™ Viral DNA/RNA Kit enables thorough and effective lysis of both solid and liquid samples. For samples with a solid matrix, 0.1 mm Glass Bead Tubes are employed to facilitate robust extraction of tough microorganisms during the bead-beating step. In contrast, samples like water, where dispersion is unnecessary, can undergo virus extraction through chemical lysis alone.

The nucleic acids purified with this kit are eluted in RNase-free water, ensuring their integrity and suitability for various downstream applications. Whether it’s PCR, CDNA synthesis, RT-qPCR, RNA-seq, or Sanger sequencing, the resulting DNA and RNA are ready for immediate use, providing researchers with reliable and reproducible results.

In summary, the VersaKit™ Viral DNA/RNA Kit offers a robust solution for researchers working with challenging samples, providing fast and reliable extraction of high-quality nucleic acids essential for a wide range of molecular biology applications.

Kit Contents

● Silica Spin Filter Columns
● Chemical lysis buffer
● Bead-beating tubes (0.1 mm glass)
● Nuclease-free water
● Elution buffer
● Collection tubes

Technical Parameters​

● Throughput: 1-24 samples
● Binding capacity: Up to 40 µg per spin filter
● Processing: Chemical lysis and/or bead-beating
● Storage temperature: Room temperature (15-30°C)
● Bead size: 0.1 mm glass (optional)
● Time per run or per prep: 45 minutes
● Sample types: Processed wastewater, stool, biosolids, and gut
material

Applications

● PCR
● cDNA synthesis
● RT-qPCR
● RNA-seq
● Sanger sequencing
● Molecular biology research involving challenging sample types

Product FAQs
Can this kit effectively purify nucleic acids from stool samples?

Yes, the VersaKit™ Viral DNA/RNA Kit is optimized for isolating nucleic acids from stool samples, ensuring efficient removal of PCR inhibitors and yielding high-quality DNA and RNA suitable for downstream applications.

What is the storage temperature for the kit components?

The kit components can be stored at room temperature (15-30°C) for convenient long-term storage and easy access during experiments.

Can I skip the bead-beating step for certain sample types?

Yes, for samples like water that do not require dispersion, the bead-beating step may be skipped, and virus extraction can be achieved through chemical lysis alone.

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