Obtain sequencing-grade plasmid DNA rapidly with the VersaKit™ Mini Lysis, featuring a one-step lysis procedure and ready-to-use DNA in just 9 minutes.
The VersaKit™ Mini Lysis offers a spin-column-based method for isolating high-quality plasmid DNA suitable for molecular biology applications. With a rapid one-step lysis procedure, bacterial cell resuspension, lysis, and DNA binding are achieved in a single buffer, reducing protocol steps and hands-on time. This kit provides sequencing-grade plasmid DNA quickly and efficiently with minimal handling. The kit includes 250 Mini Spin Columns, along with essential reagents, buffers, and lysis and collection tubes (2 mL) for streamlined DNA purification. Its innovative design allows for the isolation of up to 15 µg of plasmid DNA from just 1.5 mL of bacterial culture, making it ideal for laboratories requiring high yields of DNA for downstream applications.
● Mini Spin Columns
● Reagents
● Buffers
● Lysis and Collection Tubes (2 mL)
● Applications: Automated sequencing, PCR, restriction analysis,
cloning, etc.
● Time per run or prep per run: 9 min
● Culture volume/starting material: 1.5 mL culture volume
● Plasmid type: High-copy, cosmid DNA
● Elution volume: 50 µl
● Samples per run (throughput): 1–24 samples per run
● Technology: QuickLyse technology
● Yield: Up to 15 µg
● Processing: Manual (centrifugation)
● Automated sequencing
● PCR
● Restriction analysis
● Cloning
The kit offers ready-to-use plasmid DNA in just 9 minutes.
Up to 15 µg of high-quality plasmid DNA can be obtained from 1.5 mL of bacterial culture.
It is suitable for automated sequencing, PCR, restriction analysis, cloning and more.
MiniLysis technology combines enzymatic and osmotic processes to lyse bacterial cells in a single, 3-minute step, reducing handling and saving time.
Quantity | 250 spin columns |
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