Purify and enrich bacterial microbiome DNA from swabs and body fluids with effective host DNA depletion, optimized lysis methods, and ultra-clean columns.
VersaKit™ Microbiome DNA
Price range: $352.00 through $836.00
Purchase this item and get Product Description
The VersaKit™ Microbiome DNA revolutionizes the extraction and enrichment of bacterial microbiome DNA from diverse sample types, ranging from clinical swabs to bodily fluids. With an emphasis on maximizing bacterial DNA yield and purity, this kit incorporates innovative technologies and optimized protocols to ensure superior performance in microbiome analysis. At the heart of the VersaKit™ Microbiome DNA is its ability to selectively deplete host DNA while preserving and enriching bacterial DNA content. This feature is essential for metagenomic studies, where the overwhelming presence of host DNA can obscure microbial signatures. Through a combination of differential lysis of host cells and enzymatic degradation of host DNA, the kit efficiently removes background noise, allowing for a clearer picture of the microbial community. Moreover, the kit employs an advanced mechanical and chemical lysis process, meticulously designed to disrupt bacterial cells while minimizing bias introduced during sample preparation. By carefully balancing lysis conditions, the kit ensures optimal recovery of DNA from a wide range of bacterial species, regardless of cell wall morphology or composition. The inclusion of Ultra Clean spin columns further enhances the kit’s performance by reducing the risk of contamination and background noise. These columns undergo a rigorous decontamination process, ensuring that purified DNA remains free from external contaminants, thus preserving the integrity of downstream analyses. With its versatile applications, the VersaKit™ Microbiome DNA empowers researchers to explore the intricacies of microbial communities with unprecedented depth and accuracy. From whole genome sequencing to highly sensitive 16S rDNA-based microbiome analysis and metagenomic shotgun sequencing studies, this kit delivers reliable and reproducible results, driving discoveries in microbiome research and beyond.
Kit Contents
● Ultra Clean Mini Columns (50)
● Lysis Tubes (50)
● Buffers and reagents
● Collection Tubes (2 mL)
Technical Parameters
● Sample Input: Swabs, body fluids
● Yield: Consistent and high yields of purified DNA
● Elution Volume: Flexible elution volumes
● Processing: Manual or automated with Avam® AutEx™ Platform
● Technology: Ultra Clean spin columns
Applications
● Whole genome sequencing analysis
● Highly-sensitive 16S rDNA-based microbiome analysis
● Metagenomic shotgun sequencing studies
● PCR, digital PCR, and other molecular biology techniques
Product FAQs
What is the feature that distinguishes the VersaKit™ Microbiome DNA from other kits?
One of the key features of the VersaKit™ Microbiome DNA is its effective depletion of host DNA during the purification process, which maximizes bacterial DNA coverage in next-generation sequencing analysis.
Can the VersaKit™ Microbiome DNA be used for whole genome sequencing analysis?
Yes, the purified bacterial microbiome DNA from the VersaKit™ Microbiome DNA can be used for whole genome sequencing analysis, as well as for highly-sensitive 16S rDNA-based microbiome analysis and metagenomic shotgun sequencing studies.
Does the kit involve any enzymatic or chemical steps for cell lysis?
Yes, the VersaKit™ Microbiome DNA utilizes optimized mechanical and chemical lysis methods to effectively disrupt bacterial cells and release DNA for purification.
What downstream applications can be performed using DNA purified with the VersaKit™ Microbiome DNA?
DNA purified with this kit can be used in various downstream applications, including PCR, digital PCR, next-generation sequencing (NGS), and other molecular biology techniques.
Is the kit compatible with automated systems?
Yes, the VersaKit™ Microbiome DNA can be used manually or automated with the Avam® AutEx™ Platform, providing flexibility in sample processing
What is the expected yield of DNA from the kit?
The kit is optimized to provide consistent and high yields of purified DNA suitable for downstream applications, ensuring reliable and reproducible results.
Are there any special storage conditions for the kit components?
It is recommended to store the kit components according to the instructions provided in the user manual to maintain their stability and performance over time.
| Preps/Lysis Tubes Count | 50 preps, 50 tubes |
|---|
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