Purify high-quality DNA from formalin-fixed, paraffin-embedded tissue sections rapidly and efficiently using the VersaKit™ FFPE Tissue DNA.
VersaKit™ FFPE Tissue DNA
$343.00
Purchase this item and get Product Description
The VersaKit™ FFPE Tissue DNA is specifically engineered for the: purification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. This kit employs specialized lysis conditions to release DNA from tissue sections while overcoming inhibitory effects caused by formalin crosslinking of nucleic acids. Utilizing the Mini Elution spin columns, the kit ensures the purification of high-quality DNA in small volumes. Moreover, the DNA purification process can be seamlessly automated on the Avam® AutEx™ Platform.
Kit Contents
● Mini Elution Columns
● Avam® Proteinase K
● Buffers
● Collection Tubes (2 mL)
Technical Parameters
● Sample Type: Formalin-fixed, paraffin-embedded (FFPE) tissue
sections
● Elution Volume: 20–100 µl
● Format: Spin column
● Sample Amount: Tissue sections, each with a thickness of 5–10 µm,
for a total volume of 4 mm³
● Technology: Silica-based membrane
● Processing: Manual or automated with the Avam® AutEx™ Platform
● Applications: PCR, digital PCR, next-generation sequencing (NGS)
Applications
The purified DNA obtained using the VersaKit™ FFPE Tissue DNA is
suitable for a broad range of downstream applications, including single
nucleotide polymorphism (SNP) and short-tandem repeat (STR) genotyping,
pharmacogenomic research, and PCR.
Product FAQs
Are there any specific storage conditions for the kit components?
It is recommended to store the kit components at the specified temperatures indicated in the user manual to maintain their integrity and performance.
Can DNA isolated from FFPE samples be used for next-generation sequencing (NGS)?
Yes, the DNA purified with the VersaKit™ FFPE Tissue DNA can be used for NGS applications. However, it’s essential to consider the specific requirements of your NGS workflow and adjust protocols accordingly.
What is the typical yield of DNA obtained with this kit?
The yield of DNA extracted using the VersaKit™ FFPE Tissue DNA can vary depending on the sample type and processing conditions. However, consistent and high-quality DNA yields are achievable for downstream applications.
Does the kit include all necessary reagents and consumables for the purification process?
Yes, the kit contains all essential components, including columns, buffers, proteinase K, and collection tubes, required for the efficient purification of DNA from FFPE tissue samples.
| Quantity | 50 preps |
|---|
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