The VeraScript™ RT procedure enables sensitive detection of as few as 10 copies of template, with high cDNA yields facilitated by the high-affinity enzyme. Additionally, the process eliminates the need for an additional RNase H digestion step and ensures a fast and easy procedure devoid of tedious pipetting steps.
VeraScript™ RT Kit
Price range: $387.00 through $1,507.00
Purchase this item and get Product Description
The VeraScript™ Reverse Transcriptase (RT) Kit is crafted for highly sensitive applications involving small RNA quantities (<50 ng), making it ideal for tasks like single-cell RT-PCR and biopsy analysis. It offers exceptional efficiency across a wide dynamic range, facilitating sensitive RT-PCR even with low-copy transcripts, while effectively reading through complex RNA secondary structures without requiring adjustments. Ensuring lot-to-lot reproducibility, its components are guaranteed RNase-free, while the kit streamlines reverse transcription without the need for tedious optimization. For viral RNA, VeraScript™ RT Kit is often favored due to the presence of carrier RNA in most viral RNA preparations
Kit Contents
For 50 reactions VeraScript™ Reverse Transcriptase (1 X 0.15mL)
10X RT Buffer
100 μι dΝΤΡ Mix
Nuclease-Free Water (1 X 1.1mL)
Technical Parameters
CDNA Synthesis Procedure: RNA Isolate-> Mix enzyme, buffer, dNTPs, water minutes at and template, cDNA synthesis at 37°C for 60 minutes -> End the reaction. RNA concentration range: Can reverse transcribe RNA transcripts as low as 50 ng
Applications
VeraScript™ RT Kit is suitable for the following applications:
●Reverse Transcription of RNA templates
● RT-PCR
● qRT-PCR
● Differential display RT-PCR
●Primer extension and RACE analysis
● CDNA for cloning
Product FAQs
Does the kit come with an RNase inhibitor?
No, the kit does not come pre-packaged with an RNase inhibitor. It is recommended to use an RNase inhibitor while synthesizing cDNA with this kit.
What types of RNA can this kit reverse transcribe?
All types of RNA targets in an RNA isolate mixture can be used for reverse transcription using this kit.
How stable is the cDNA synthesized by this kit?
This unique kit guarantees stable cDNA outputs if stored at -20°C.
Does this kit have an integrated polymerase?
No, this kit cannot perform direct PCR reactions. The kit is only for cDNA synthesis.
| Reactions | 200, 50 |
|---|
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