The TaqType™ Mutation Detect PCR Kit (200) is designed to deliver time-effective and reliable detection of anomalies like translocations, insertions, and deletions. This kit is based on a proprietary buffer system as well as FusionTaq™ Pro DNA Polymerase with an optimized protocol for reliable results.
TaqType™ Mutation Detection PCR Kit
$302.00
Purchase this item and get Product Description
The TaqType™ Mutation Detect PCR Kit is a highly efficient and time-effective solution designed for the reliable detection of mutations. Utilizing a patented buffer system and FusionTaq™ Pro DNA Polymerase, the kit ensures specific and robust amplification of mutant loci through multiplex PCR reactions with minimal optimization. The kit’s dedicated protocols and optimized components enable straightforward analysis on various platforms, including agarose gels, automated electrophoresis instruments, and high-resolution capillary sequencing. It outperforms other kits, guaranteeing reproducible results in the analysis of mutations, making it especially suitable for genetically modified organisms or microbes.
The kit is made to be integrated with the AvBlend™ Solution additive and the GlowSight™ Dye to improve primer annealing and direct gel loading of PCR products.
The TaqType™ Mutation Detect PCR Kit is versatile, functioning as both a reliable genotyping tool and a preamplification method for commercially available SNP detection PCR-based systems. With its high-plex grade multiplex capability, the kit reduces time and costs, making it an ideal choice for simultaneous amplification of multiple mutation targets. The user-friendly design, including room temperature setup and optimized programs, enhances convenience without compromising accuracy in mutation detection and SNP analysis.
Kit Contents
For 200 reactions
● TaqType™ Multiplex PCR Master Mix
● 5X AvBlend™ Solution
● GlowSight™ Loading Dye
● Nuclease-Free Water (2 X 1.9 mL)
Technical Parameters
● MasterMix Format
● Highly specific amplifications of SNPs for mutation detection
● Room temperature reaction setup
Applications
For research applications to detect insertions, deletions, duplications and
translocations from
● Disease loci
● GMO products
● Transgenics
Product FAQs
What downstream analysis platforms are compatible with this kit?
Widely available commercial platforms with applications for sequencing etc. are compatible with PCR products from this kit
Can the GlowSight™ Dye be used with capillary sequencers?
No, GlowSight™ dye is not compatible with capillary sequencers.
How is primer annealing efficiency ensured?
The PrimeFactor™ additive present in the AvBlend™ Solution increases local concentration of primers around the template and also aids in stabilization of the primers post-annelaing, therefore permitting Fusion Taq™ to extend effectively as compared to other Taq Polymerases on the market.
Is the kit suitable for the detection of genetically modified organisms or microbes?
Yes, one of the prime applications of this kit is the detection of mutations in GMO products and cloned microbes.
| Reactions | 200 |
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