TaqSense™ HotStart DNA Polymerase Kit enables efficient DNA synthesis with hot-start capability, minimizing non-specific amplification and primer-dimers, ideal for demanding PCR applications.
TaqSense™ HotStart DNA Polymerase
Price range: $104.00 through $1,067.00
Purchase this item and get Product Description
TaqSense™ HotStart DNA Polymerase Kit offers a reliable solution for high-fidelity DNA amplification in PCR applications. Combining the robust enzymatic activity of recombinant Taq DNA polymerase with the precision of a monoclonal antibody-based hot-start system, this kit ensures efficient and specific amplification of target DNA sequences.
This advanced formulation enables easy setup of PCR reactions at room temperature, thanks to the reversible binding of the monoclonal antibody to the enzyme. By inhibiting polymerase activity during initial setup, TaqSense™ HotStart minimizes non-specific amplification and primer-dimer formation, leading to cleaner PCR results with reduced background noise. The antibody dissociates from the polymerase during the PCR cycling process, eliminating the need for additional activation steps and streamlining the PCR workflow. TaqSense™ HotStart DNA Polymerase exhibits 5′-3′ exonuclease activity without 3′–5′ exonuclease activity, ensuring accurate DNA synthesis and minimizing the risk of sequence errors. With its ability to amplify DNA fragments up to 5 kb in length, this kit is suitable for a wide range of PCR applications, including those involving GC-rich templates or challenging specimens.
The kit includes TaqSense™ HotStart DNA Polymerase, 10x TaqSense™ HotStart Reaction Buffer, and 50 mM MgCl₂, providing researchers with all the necessary components for reliable and reproducible PCR amplification. Whether performing hot-start PCR, real-time PCR, multiplex PCR, or amplifying difficult templates, TaqSense™ HotStart DNA Polymerase Kit delivers consistent and high-quality results, making it an essential tool for molecular biology laboratories.
Kit Contents
● TaqSense™ HotStart DNA Polymerase (5 U/µL)
● 10x TaqSense™ HotStart Reaction Buffer
● 50 mM MgCl2
Technical Parameters
● Storage temperature: -20°C
● Unit Definition: The amount of enzyme required to incorporate 10 nmol of dNTPs into an insoluble DNA fraction in 30 minutes at 75°C in a 50 μL reaction.
Applications
● Hot-start and real-time PCR
● Singleplex and multiplex PCR
● PCR with DNA from various specimens
● Specific amplification of difficult templates (GC-rich)
Product FAQs
What is the advantage of using TaqSense™ HotStart DNA Polymerase for PCR?
TaqSense™ HotStart DNA Polymerase offers hot-start capability, allowing for more specific amplification by minimizing non-specific products and primer-dimers. It simplifies PCR setup and is ideal for demanding PCR applications requiring high specificity.
Can TaqSense™ HotStart DNA Polymerase be used for real-time PCR?
Yes, TaqSense™ HotStart DNA Polymerase is suitable for real-time PCR, providing robust performance and high sensitivity in quantitative PCR assays.
How does the hot-start feature of TaqSense™ HotStart DNA Polymerase work?
The monoclonal antibody in TaqSense™ HotStart DNA Polymerase binds to the enzyme, inhibiting polymerase activity at room temperature. This prevents non-specific primer annealing and primer-dimer formation during PCR setup, enhancing specificity and reducing background noise.
| Quantity | 200U, 2500U, 500U |
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