TaqBase™ PCR Foundation Kit is masterfully crafted in a 2X format to simplify your PCR setups by reducing the risk of contamination, minimizing pipetting steps & optimization time with reproducible and accurate results.
TaqBase™ PCR Foundation Kit
Price range: $179.00 through $693.00
Purchase this item and get Product Description
The TaqBase™ PCR Foundation Kit offers a comprehensive solution for robust PCR performance, providing essential components including TaqBase™ DNA Polymerase, OptiMix™ PCR Buffer, dNTP mix, and AVBlend™. Specifically designed for challenging templates, the kit ensures efficient amplification even with low-copy targets or GC-rich sequences. Rigorous quality control measures guarantee consistent results, with Taq DNA Polymerase subjected to extensive testing for specificity and reproducibility. The innovative OptiMix™ PCR Buffer minimizes the need for optimization, enabling specific PCR across various conditions. The GlowSight™ PCR Buffer enhances pipetting visibility and facilitates direct gel loading, simplifying workflow and expediting results. Additionally, the kit features an optimized, user-friendly protocol for convenient and reliable PCR setup. AVBlend™ is included to address suboptimal PCR conditions, enhancing versatility and performance.
Trusted by scientists worldwide, the TaqBase™ PCR Foundation Kit is a cornerstone in molecular biology laboratories for its exceptional performance and convenience.
Kit Contents
For 250 Units
● TaqBase™ DNA Polymerase (250 units)
● 10X Optimix™ PCR Buffer
● 10X GlowSight™ PCR Buffer
● 5X AVBlend™
● 25 mM MgCl2
● dNTP Mix
Technical Parameters
● MgCl2 Optimization: Includes 25 mM MgCl2 solution for optimized reaction conditions, allowing for precise control over PCR performance.
● Buffer Composition: The kit features a ready-to-use PCR buffer with optimized pH and ionic strength to ensure reliable amplification.
● Amplification Range: Suitable for amplifying DNA fragments up to 5 kb, ideal for standard PCR applications.
● Sensitivity: Capable of detecting low DNA quantities due to its high amplification efficiency.
● Compatibility: Works with a wide range of thermal cyclers and templates, ensuring flexibility across different platforms.
Applications
Optimal for the following applications:
● DNA fingerprinting
● RT-PCR
● General PCR
● Screening
Product FAQs
Does the GlowSight™ PCR Buffer compromise the PCR reaction in any way?
The GlowSight™ PCR buffer offers the same specificity and precision offered by the OptiMix™ PCR Buffer affording users an additional use-case along with the minimal optimization and increasing product loading visibility without the need of an another gel loading dye.
Does this polymerase promote secondary structure formation?
No, the TaqBase™ DNA Polymerase when used with AVBlend™ actively discourages the formation of secondary structures due to its capability of amplifying GC-rich templates.
What advantages does the AVBlend™ offer?
The unique property of dual-cation mix in the AVBlend™ offers a specific and high affinity binding of the primer sets at the target sites
Is it mandatory to use the GlowSight™ Buffer?
The use of GlowSight™ Buffer is optional, however, the use of GlowSight™ is recommended where restriction digestion reactions or genetic screening applications are involved for greater convenience and quick results.
| Reactions | 1000, 250 |
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