TagPurge™ Histidine Removal Kit

TagPurge™ Histidine Removal Kit

Price range: $645.00 through $652.00

The TagPurge™ Histidine Removal Kit is an efficient enzymatic system for removing N-terminal His tags from recombinant proteins for structural biology applications.

Purchase this item and get 64-65 Points
Purchase this item and get 64-65 Points
Product Description

Facilitate efficient and specific removal of N-terminal His tags from recombinant proteins with the TagPurge™ Histidine Removal Kit, featuring the DAPase Enzyme. Engineered for high specificity and activity, this enzyme enables the sequential cleavage of dipeptides from His tags, ensuring >95% removal in just 30 minutes at 37°C. The system is optimized for use with proteins containing intrinsic DAPase stop points, expressed using the TagPurge™ vector. With sufficient enzyme for processing up to 10 mg of tagged protein, the TagPurge™ Histidine Removal Kit guarantees high-purity end products, essential for downstream applications such as protein structure determination by NMR or X-ray crystallography, as well as the production of therapeutic proteins. Unlock the full potential of your His-tagged proteins with the TagPurge™ Histidine Removal Kit for precise and reliable His-tag removal.

Kit Contents

● DAPase Enzyme
● TagPurge™ Vector

Technical Parameters​

● Efficiency: >95% removal in 30 minutes at 37°C
● Suitable for proteins with intrinsic DAPase stop points
● Processing capacity: Up to 10 mg of tagged protein

Applications

● Protein structure determination by NMR or X-ray crystallography
● Production of therapeutic proteins

Product FAQs
What is the processing capacity of the TagPurge™ Histidine Removal Kit?

The system can process up to 10 mg of tagged protein per reaction.

What downstream applications is the TagPurge™ Histidine Removal Kit suitable for?

The system is suitable for protein structure determination by NMR or X-ray crystallography, as well as the production of therapeutic proteins.

How long does it take to remove the His tag using the TagPurge™ Histidine Removal Kit?

The TagPurge™ Histidine Removal Kit achieves >95% His-tag removal in just 30 minutes at 37°C.

Is the TagPurge™ Histidine Removal Kit compatible with proteins expressed using other vectors?

The system is optimized for use with proteins containing intrinsic DAPase stop points, expressed using the TagPurge™ PQE-2 vector.

Can the TagPurge™ Histidine Removal Kit be used for large-scale protein purification?

Yes, the system is suitable for both small-scale and large-scale protein purification processes.

Quantity

2.5U, 25µg of pQE-2 vector
SKU: PA1845 Category:
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