The SlingShot™ RT-PCR Kit consists of ScriptSwift™ Reverse Transcriptases as well as VeraScript™ and a proofreading enzyme. It has a different two-phase hot-start procedure for room-temperature setup. The enzymes present increase specificity, sensitivity, and fidelity. Visual pipetting control results in fewer pipetting errors. It is also faster than ever by 1-hour cycling protocol. Additionally, tracing dyes are included to monitor the loading process, further minimizing pipetting errors.
SlingShot™ RT-PCR Kit
Price range: $253.00 through $7,916.00
Purchase this item and get Product Description
The SlingShot™ RT-PCR Kit is a cutting-edge solution for highly sensitive and specific one-step RT-PCR. This kit boasts a unique two-phase hot-start procedure for room-temperature setup, reducing pipetting errors through visual control. With a rapid 1-hour cycling protocol, it outpaces traditional methods. The kit features ScriptSwift™ and VeraScript™ Reverse Transcriptases, equipped with an RT-blocker for ambient temperature inactivation, ensuring precise reverse transcription. For PCR amplification, a trio of DNA polymerases, including FusionTaq™ and high-fidelity proofreading enzymes, ensures superior specificity and accuracy. Tracing dyes aid in monitoring the loading process, minimizing errors. The master mix, containing an innovative dual-cation OptiMix™ PCR buffer, facilitates co-amplification of an internal positive control, while AVBlend™ enhances efficiency with challenging templates. Room-temperature setup convenience and integrated control features make it suitable for automated workflows. The SlingShot RT-PCR Kit is your go-to for easy, sensitive, and error-resistant one-step RT-PCR, applicable to various RNA templates, making it ideal for applications like virus detection, molecular diagnostics research, and gene expression analysis. Experience faster and easier RT-PCR setup with this all-inclusive kit, delivering reliable results in a streamlined, user-friendly manner.
Kit Contents
For 200 reactions:
● SlingShot™ RT-PCR Master Mix (2 x 1 mL)
● SlingShot™ RT Mix (1 x 0.2 mL)
● Template Guide (1 x 0.2 mL)
● MasterMix Guide (1 x 0.05 mL)
● Water (2 x 1.9mL)
● AVBlend™ (1 x 2 mL)
Technical Parameters
Amplification of a wide range of RNA template concentrations from 0.1 pg to 2 µg. Very high sensitivity. High-fidelity proofreading polymerase. Can be used to perform PCR with two primer pairs simultaneously. Gel tracking on gel using orange dye (50 bp) RNase Inhibition Amplicons of up to 4kb.
Applications
The SlingShot™ RT-PCR Kit is suitable for the following applications: Viral detection Gene expression analysis.
Product FAQs
What makes the SlingShot™ RT-PCR Kit unique?
The kit features a two-phase hot-start procedure for room-temperature setup, which reduces pipetting errors, and offers a 1-hour cycling protocol for faster results.
What enzymes are included in the kit and how do they contribute to specificity and sensitivity?
The kit includes ScriptSwift™ and VeraScript™ Reverse Transcriptases with an RT-blocker for ambient temperature inactivation, ensuring precise reverse transcription. Three DNA polymerases, including a proofreading enzyme, enhance specificity and accuracy during PCR amplification.
What is the significance of the two-phase hot-start procedure in the kit?
The hot-start procedure eliminates nonspecific amplification products, such as primer-dimers, by activating DNA polymerases and simultaneously inactivating reverse transcriptases at 95°C.
What is the role of tracking dyes in the SlingShot™ RT-PCR Kit
Tracking dyes aid in monitoring the loading process, minimizing pipetting errors during setup.
| Reactions | 200, 2000, 50 |
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