SeqVersa™ rRNA Depletion Kit

The SeqVersa™ rRNA Depletion Kit is a comprehensive solution designed to improve RNA sequencing workflows by selectively depleting highly abundant globin mRNA and rRNA molecules from human, mouse, and rat total RNA samples. These molecules can obscure less abundant transcripts in RNA sequencing experiments, affecting data quality and sensitivity. By removing them, the kit enables deeper coverage and enhanced detection of low-abundance transcripts, critical for comprehensive transcriptomic analyses.

This kit employs a dual enzymatic approach for efficient removal of globin mRNA and rRNA, ensuring minimal impact on RNA integrity and quality. The depletion process is robust and highly specific, significantly reducing the representation of these abundant transcripts without the need for poly(A) selection. This approach preserves the representation of non-polyadenylated RNA species, such as long non-coding RNAs (IncRNAs) and small nucleolar RNAS (snoRNAs), which are essential for many biological processes and disease studies.

Included in the kit are RNA sample purification beads, which facilitate the rapid and efficient cleanup of depleted RNA samples, minimizing hands-on time and optimizing downstream library preparation. The purified RNA is suitable for a wide range of applications, including RNA sequencing (RNA-seq), small RNA sequencing, and other transcriptomic analyses.

The SeqVersa™ rRNA Depletion Kit is compatible with various input amounts of total RNA, making it versatile for different experimental needs. Whether studying gene expression profiling in clinical samples or investigating complex biological pathways, this kit offers researchers a reliable tool to enhance the quality and depth of RNA sequencing data.