SeqVersa™ RNA Fragmentation Kit offers precise and efficient RNA fragmentation for next-generation sequencing (NGS) library preparation, ensuring uniform fragment sizes for optimal sequencing performance.
SeqVersa™ RNA Fragmentation Kit
$85.00
Purchase this item and get Product Description
SeqVersa™ RNA Fragmentation Kit is designed to provide consistent and controlled RNA fragmentation, a crucial step in the preparation of high-quality RNA sequencing libraries. Fragmentation of RNA is essential for creating uniform fragment sizes, which contributes to efficient and accurate sequencing on various NGS platforms.
The kit utilizes magnesium ions to induce RNA fragmentation, a method known for its precision and reproducibility. This chemical approach ensures that RNA is broken into appropriately sized fragments, suitable for subsequent library preparation steps. By controlling the fragmentation process, researchers can optimize the quality and consistency of their RNA sequencing libraries.
Key features of the SeqVersa™ RNA Fragmentation Kit include: Controlled Fragmentation: The kit allows precise control over the fragmentation process, ensuring uniform RNA fragment sizes that are ideal for sequencing.
Reproducibility: The use of magnesium ions for RNA fragmentation offers high reproducibility, which is critical for generating consistent results across different experiments and samples.
Ease of Integration: The kit is compatible with standard RNA library preparation workflows, allowing seamless integration into existing protocols and ensuring efficient sample processing.
The SeqVersa™ RNA Fragmentation Kit is suitable for a wide range of applications, including transcriptome analysis, gene expression studies, and RNA-Seq experiments. It provides researchers with the tools needed to produce high-quality RNA sequencing libraries, facilitating accurate and comprehensive analysis of RNA samples.
Kit Contents
● RNA Fragmentation Buffer with Magnesium
● Fragmentation Enzyme Mix
Technical Parameters
● Fragmentation Method: Magnesium ion-induced RNA fragmentation
● Fragment Size Control: Optimized for uniform RNA fragment sizes
● Compatibility: Suitable for various RNA-Seq library preparation workflows
● Storage Conditions: -20°C for all reagents
Applications
● RNA sequencing (RNA-Seq)
● Transcriptome analysis
● Gene expression studies
● Preparation of high-quality RNA sequencing libraries
Product FAQs
What is the SeqVersa™ RNA Fragmentation Kit used for?
The kit is used for controlled and efficient fragmentation of RNA, a crucial step in the preparation of high-quality RNA sequencing libraries.
How does the SeqVersa™ RNA Fragmentation Kit ensure uniform fragment sizes?
The kit uses magnesium ions to induce precise RNA fragmentation, ensuring that the resulting RNA fragments are uniform in size and suitable for sequencing.
Can the SeqVersa™ RNA Fragmentation Kit be integrated into existing RNA library preparation workflows?
Yes, the kit is designed to be compatible with standard RNA library preparation protocols, allowing for seamless integration and efficient sample processing.
What are the benefits of using magnesium ions for RNA fragmentation?
Magnesium ion-induced fragmentation offers high reproducibility and precise control over fragment sizes, which is essential for generating. consistent and high-quality RNA sequencing libraries
What are the storage requirements for the SeqVersa™ RNA Fragmentation Kit components?
All components should be stored at -20°C to maintain their stability and performance, as recommended in the user manual.
Is the SeqVersa™ RNA Fragmentation Kit suitable for all types of RNA-Seq experiments?
Yes, the kit is versatile and suitable for various RNA-Seq applications, including transcriptome analysis and gene expression studies, providing researchers with the flexibility to explore different aspects of RNA biology.
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