The SeqVersa™ RNA First Strand Synthesis Kit provides a reliable and efficient solution for synthesizing first-strand cDNA from RNA templates. Designed for use in next-generation sequencing and other molecular biology applications, it ensures high yield and reproducibility.
SeqVersa™ RNA First Strand Synthesis Kit
Price range: $174.00 through $555.00
Purchase this item and get Product Description
The SeqVersa™ RNA First Strand Synthesis Kit is meticulously designed to meet the needs of researchers performing RNA-seq and other transcriptomic analyses. This kit facilitates the synthesis of first-strand cDNA from RNA templates, providing a critical step in the preparation of high-quality cDNA libraries for next-generation sequencing (NGS).
The SeqVersa™ kit employs an optimized reverse transcriptase enzyme blend that ensures robust and efficient synthesis of cDNA. This high-efficiency enzyme system is coupled with a proprietary buffer formulation that enhances enzyme stability and performance across a wide range of RNA inputs and qualities. Whether working with high-quality RNA or challenging samples such as FFPE-derived RNA, this kit delivers consistent and reliable results.
With a streamlined protocol, the SeqVersa™ RNA First Strand Synthesis Kit minimizes hands-on time and simplifies workflow, making it suitable for high-throughput sequencing projects. The kit is compatible with both poly(A)-selected and ribosomal RNA-depleted RNA, allowing flexibility in experimental design.
The high-fidelity synthesis ensures an accurate representation of the transcriptome, capturing both abundant and rare transcripts with minimal bias. This makes the SeqVersa™ kit ideal for diverse applications, including differential gene expression analysis, transcript discovery, and RNA modification studies.
Kit Contents
● Enzymatic Fragmentation Mix
● End Repair and dA-Tailing Mix
● Adapter Ligation Mix
● Sample Purification Beads
● Library Amplification Primers (optional)
● Nuclease-Free Water
Technical Parameters
● Input RNA Quantity: 10 pg – 1 μg
● Reaction Volume: 20 μl
● Reaction Time: 1 hour
● Storage Conditions: -20°C
● Compatibility: Compatible with both poly(A)-selected and ribosomal RNA-depleted RNA
Applications
● RNA sequencing (RNA-seq)
● Gene expression analysis
● Transcriptome profiling
● cDNA library preparation for NGS
● Analysis of RNA modifications
Product FAQs
What types of RNA can be used with the SeqVersa™ RNA First Strand Synthesis Kit?
The kit is compatible with a wide range of RNA types, including poly(A)-selected RNA and ribosomal RNA-depleted RNA, making it versatile for various transcriptomic studies.
What is the recommended input RNA quantity for optimal results?
The kit works effectively with RNA inputs ranging from 10 pg to 1 μg, accommodating both low- and high-input experiments.
How long does the first strand cDNA synthesis process take?
The first strand synthesis reaction takes approximately 1 hour to complete, allowing for rapid processing of multiple samples.
Can this kit be used with degraded RNA samples, such as those from FFPE tissues?
Yes, the SeqVersa™ RNA First Strand Synthesis Kit is designed to perform well with challenging samples, including degraded RNA from FFPE tissues, ensuring reliable cDNA synthesis.
| Reactions | 24 reactions, 96 reactions |
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