The SeqVersa™ Poly-A mRNA Isolation Kit offers a highly efficient method for isolating polyadenylated mRNA from total RNA, ensuring high purity and yield. This kit is ideal for preparing mRNA samples for downstream applications such as next-generation sequencing (NGS) and quantitative PCR (qPCR).
SeqVersa™ Poly-A mRNA Isolation Kit
Price range: $656.00 through $2,495.00
Purchase this item and get Product Description
The SeqVersa™ Poly-A mRNA Isolation Kit is meticulously designed to provide researchers with a robust and efficient method for isolating polyadenylated mRNA from total RNA samples. Poly-A mRNA represents the coding portion of the transcriptome, making its isolation crucial for various genomic and transcriptomic studies. This kit leverages oligo(dT) beads to selectively capture and purify mRNA with polyadenylated tails, ensuring high specificity and yield.
The optimized protocol minimizes RNA degradation and contamination, providing high-quality mRNA suitable for a range of downstream applications, including next-generation sequencing (NGS), quantitative PCR (qPCR), and cDNA library construction. The SeqVersa™ Poly-A mRNA Isolation Kit is designed to handle high-input RNA samples, making it ideal for projects requiring significant amounts of starting material, such as tissue samples or cell cultures.
Key features include a streamlined workflow, high reproducibility, and compatibility with various downstream processing steps. Researchers can trust this kit for consistent and reliable isolation of poly-A mRNA, enabling accurate and meaningful data generation in transcriptomic and genomic studies.
Kit Contents
● Oligo(dT) Beads
● Binding Buffer
● Washing Buffers
● Elution Buffer
● RNase-free Water
Technical Parameters
● Input RNA Quantity: Up to 1 mg
● Yield: >80% of poly-A mRNA
● Processing Time: Approximately 2 hours
● Storage Conditions: 4°C (Oligo(dT) Beads), -20°C (Buffers)
● Compatibility: Compatible with various RNA samples (e.g., total RNA from tissues, cells)
Applications
● Isolation of poly-A mRNA from total RNA
● Preparation of mRNA for next-generation sequencing (NGS)
● Quantitative PCR (qPCR) analysis
● cDNA library construction
● Transcriptomic and genomic studies
Product FAQs
What is the maximum amount of total RNA that can be processed with the SeqVersa™ Poly-A mRNA Isolation Kit?
The kit can handle up to 1 mg of total RNA, making it suitable for high-input samples.
How long does the mRNA isolation process take?
The entire isolation process takes approximately 2 hours, from binding to elution.
Is this kit compatible with RNA from various sources, such as tissues and cell cultures?
Yes, the SeqVersa™ Poly-A mRNA Isolation Kit is compatible with RNA from a wide range of sources, including tissues and cell cultures.
What downstream applications can benefit from using the isolated poly-A mRNA?
The isolated poly-A mRNA is ideal for next-generation sequencing (NGS), quantitative PCR (qPCR), cDNA library construction, and other transcriptomic and genomic studies.
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