The SeqVersa™ PCR Free FS DNA Library Prep Kit enables the preparation of high-quality, fragmentation-free DNA libraries without PCR amplification, ensuring accurate and unbiased sequencing results.
SeqVersa™ PCR Free FS DNA Library Prep Kit
Price range: $689.00 through $2,612.00
Purchase this item and get Product Description
The SeqVersa™ PCR Free FS DNA Library Prep Kit offers an advanced solution for researchers aiming to prepare DNA libraries for sequencing without the need for PCR amplification. This kit is specifically designed to create high-quality libraries from DNA samples while avoiding the introduction of bias and artifacts that are often associated with PCR steps. The PCR-free approach ensures that the original representation of the genome is maintained, making this kit ideal for applications that demand high fidelity and precision.
This kit includes a comprehensive set of reagents for DNA fragmentation, end repair, A-tailing, adapter ligation, and sample purification. The fragmentation step is enzyme-based, which allows for precise control over the size distribution of the DNA fragments. The inclusion of sample purification beads facilitates the cleanup and concentration of the prepared libraries, streamlining the workflow and enhancing the quality of the final product.
The SeqVersa™ PCR Free FS DNA Library Prep Kit is optimized for simplicity and efficiency, with a user-friendly protocol that reduces hands-on time and minimizes potential sources of error. It is compatible with a range of DNA input amounts and is particularly suited for high-throughput sequencing platforms, such as Illumina.
Researchers using this kit can expect consistent, high-quality libraries that accurately reflect the original DNA samples, making it an essential tool for whole genome sequencing, metagenomics, and other genomic research applications.
Kit Contents
● FS Fragmentation Buffer
● FS Fragmentation Enzyme Mix
● End Repair Reaction Buffer
● End Repair Enzyme Mix
● A-Tailing Reaction Buffer
● A-Tailing Enzyme Mix
● Adapter Ligation Buffer
● Adapter Ligation Mix
● Sample Purification Beads
● Elution Buffer
Technical Parameters
● Input DNA Quantity: 100 ng to 1 µg
● Library Yield: Suitable for multiple sequencing runs
● Processing Time: Approximately 4 hours
● Storage Conditions: -20°C for all reagents
● Compatibility: Compatible with various DNA samples, including fragmented and intact genomic DNA
Applications
● Whole genome sequencing
● Metagenomics
● Genomic studies requiring high fidelity and low bias
● Any sequencing application where PCR bias needs to be avoided
Product FAQs
What is the benefit of using a PCR-free library prep kit for sequencing?
A PCR-free library prep kit reduces the risk of introducing bias and artifacts during library preparation, preserving the original genomic representation and ensuring more accurate sequencing results.
What range of DNA input is required for the SeqVersa™ PCR Free FS DNA Library Prep Kit?
The kit is designed to work with DNA inputs ranging from 100 ng to 1 µg. How long does the library preparation process take using this kit? The entire process takes approximately 4 hours.
Is the SeqVersa™ PCR Free FS DNA Library Prep Kit compatible with different types of DNA samples?
Yes, the kit is compatible with a variety of DNA samples, including both fragmented and intact genomic DNA, making it versatile for different sequencing applications.
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