SeqVersa™ Methyl Sequencing Kit

SeqVersa™ Methyl Sequencing Kit

Price range: $978.00 through $3,672.00

SeqVersa™ Methyl Sequencing Kit enables precise detection of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) in DNA, facilitating advanced epigenetic studies.

Purchase this item and get 97-367 Points
Purchase this item and get 97-367 Points
Product Description

The SeqVersa™ Methyl Sequencing Kit is an advanced tool for the comprehensive analysis of DNA methylation patterns, specifically targeting the detection of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC). This kit provides a streamlined, reliable method for converting and sequencing methylated DNA, making it an essential resource for researchers studying epigenetic modifications and their implications in gene regulation, development, and disease.

The core technology of the SeqVersa™ Methyl Sequencing Kit involves enzymatic conversion, which offers a significant advantage over traditional bisulfite conversion methods. Enzymatic conversion preserves the integrity of the DNA, resulting in higher-quality sequencing data and reduced DNA degradation. This method is highly sensitive and specific, ensuring accurate differentiation between 5mC and 5hmC, crucial for detailed epigenetic analysis.

The kit includes all necessary reagents for DNA conversion, library preparation, and sequencing, providing a comprehensive solution that integrates seamlessly into existing workflows. The protocol is designed to be user-friendly and efficient, minimizing hands-on time while maximizing throughput and data quality.

SeqVersa™ Methyl Sequencing Kit is suitable for a variety of sample types, including genomic DNA from different organisms, cultured cells, and tissue samples. Its high sensitivity allows for the analysis of low-input DNA, making it ideal for challenging samples. The generated data is compatible with major sequencing platforms, facilitating straightforward integration into bioinformatics pipelines for downstream analysis.

Kit Contents

● Enzymatic Conversion Mix
● DNA Conversion Buffer
● Library Preparation Reagents
● DNA Purification Components

Technical Parameters​

● Input DNA Quantity: 10 ng to 500 ng
● Conversion Efficiency: >99% for 5mC and 5hmC
● Library Preparation Time: Approximately 6 hours
● Storage Conditions: -20°C for all reagents
● Compatibility: Suitable for genomic DNA, cultured cells, and tissue
samples

Applications

● Whole-genome methylation analysis
● Targeted methylation sequencing
● Detection of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine(5hmC)
● Epigenetic research in gene regulation and development
● Studies on disease-related methylation patterns

Product FAQs
What amount of input DNA is required for the SeqVersa™ Methyl Sequencing Kit?

The kit is designed to work with input DNA quantities ranging from 10 ng to 500 ng, allowing flexibility for various sample types and experimental needs.

How does enzymatic conversion compare to bisulfite conversion in terms of DNA integrity?

Enzymatic conversion, as used in the SeqVersa™ Methyl Sequencing Kit, preserves DNA integrity better than traditional bisulfite conversion methods, resulting in higher-quality sequencing data with less DNA degradation.

Can this kit be used with different types of samples?

Yes, the SeqVersa™ Methyl Sequencing Kit is compatible with a variety of sample types, including genomic DNA from different organisms, cultured cells, and tissue samples.

What sequencing platforms are compatible with the data generated from this kit?

The sequencing data generated by the SeqVersa™ Methyl Sequencing Kit is compatible with major sequencing platforms, facilitating easy integration into existing bioinformatics pipelines for downstream analysis.

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