SeqVersa™ Methyl Sequencing Conversion Kit provides an efficient and reliable method for converting methylated cytosines in DNA, enabling accurate methylation analysis for epigenetic studies.
SeqVersa™ Methyl Sequencing Conversion Kit
Price range: $201.00 through $733.00
Purchase this item and get Product Description
SeqVersa™ Methyl Sequencing Conversion Kit is specifically designed for the efficient conversion of methylated cytosines (5-mC) to thymine during bisulfite treatment, an essential step for DNA methylation analysis. This kit is optimized to provide high conversion efficiency while minimizing DNA degradation, ensuring that the integrity and quantity of your DNA samples are maintained.
Accurate DNA methylation analysis is crucial for understanding epigenetic modifications and their role in gene expression regulation, disease progression, and developmental processes. SeqVersa™ Methyl Sequencing Conversion Kit delivers reliable and reproducible results, making it an ideal choice for researchers conducting epigenetic studies.
The kit includes all necessary reagents for the conversion process, and the protocol is designed to be user-friendly, allowing for straightforward implementation in your laboratory workflow. SeqVersa™ Methyl Sequencing Conversion Kit is compatible with various downstream applications, including PCR, qPCR, and next-generation sequencing (NGS), providing flexibility and versatility for your research needs.
By using the SeqVersa™ Methyl Sequencing Conversion Kit, researchers can achieve accurate and detailed methylation profiles, facilitating a deeper understanding of epigenetic modifications and their biological implications.
Kit Contents
● Conversion Reagent Mix
● DNA Denaturation Solution
● DNA Binding Buffer
● Wash Buffers
● Elution Buffer
Technical Parameters
● Conversion Efficiency: >99% for 5-mC to thymine
● DNA Recovery: High yield with minimal degradation
● Sample Input: Suitable for low to high DNA input amounts
● Storage: Store at -20°C
● Shelf Life: 12 months from the date of manufacture
● Compatibility: Suitable for PCR, qPCR, and NGS applications
Applications
● DNA methylation analysis
● Epigenetic studies
● Cancer research
● Developmental biology
● Disease progression studies
● Gene expression regulation studies
Product FAQs
What is the conversion efficiency of the SeqVersa™ Methyl Sequencing Conversion Kit?
The kit provides a conversion efficiency of greater than 99% for 5-methylcytosine (5-mC) to thymine.
How should I store the reagents included in the SeqVersa™ Methyl Sequencing Conversion Kit?
The reagents should be stored at -20°C to ensure their stability and effectiveness over time.
Is the SeqVersa™ Methyl Sequencing Conversion Kit compatible with next-generation sequencing (NGS)?
Yes, the kit is compatible with various downstream applications, including PCR, qPCR, and NGS.
Can the SeqVersa™ Methyl Sequencing Conversion Kit be used with low DNA input amounts?
Yes, the kit is suitable for a wide range of DNA input amounts, from low to high, ensuring flexibility for different sample types and quantities.
| Kit Capacity | 24 reactions, 96 reactions |
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