SeqVersa™ Low Input cDNA Synthesis & Amplification Kit

SeqVersa™ Low Input cDNA Synthesis & Amplification Kit

Price range: $755.00 through $2,563.00

The SeqVersa™ Low Input cDNA Synthesis & Amplification Kit enables efficient synthesis and amplification of cDNA from single cells or low input RNA, ensuring high-quality libraries for downstream applications such as sequencing and gene expression analysis.

Purchase this item and get 75-256 Points
Purchase this item and get 75-256 Points
Product Description

The SeqVersa™ Low Input cDNA Synthesis & Amplification Kit is designed to facilitate the generation of high-quality cDNA from single cells or low input RNA samples. This kit is ideal for researchers focusing on single-cell transcriptomics, gene expression analysis, and other applications where starting material is limited.

The kit employs an optimized workflow that integrates reverse transcription and cDNA amplification in a seamless process, ensuring minimal sample loss and maximum yield. The reverse transcription step uses a robust enzyme mix that efficiently synthesizes first-strand cDNA from low quantities of RNA. Following this, the cDNA amplification step uses a high-fidelity polymerase to produce sufficient material for downstream applications, such as sequencing or microarray analysis.

SeqVersa™ Low Input cDNA Synthesis & Amplification Kit is particularly beneficial for studies requiring high sensitivity and precision. By enabling the analysis of individual cells or small populations, this kit supports the exploration of cellular heterogeneity and rare transcripts that are often missed in bulk RNA analyses.

The protocol is user-friendly and designed to minimize hands-on time while ensuring reproducible results. With this kit, researchers can confidently perform single-cell or low-input RNA studies with the assurance of obtaining high-quality, amplified cDNA suitable for a wide range of genomic applications.

Kit Contents

● Reverse Transcription Enzyme Mix
● First Strand Synthesis Buffer
● Second Strand Synthesis Enzyme Mix
● cDNA Amplification Buffer
● High-Fidelity Polymerase Mix
● Nuclease-Free Water
● Sample Purification Beads
● Elution Buffer

Technical Parameters​

● Input RNA Quantity: Single cells or 10 pg to 1 ng of total RNA
● Library Yield: High yield suitable for sequencing and other analyses
● Processing Time: Approximately 5 hours
● Storage Conditions: -20°C for all reagents
● Compatibility: Compatible with RNA from various sources, including mammalian, plant, and microbial cells

Applications

● Single-cell transcriptomics
● Low-input RNA sequencing
● Gene expression profiling
● Analysis of rare transcripts and cellular heterogeneity
● Any application requiring high sensitivity cDNA synthesis and amplification

Product FAQs
What is the minimum RNA input required for the SeqVersa™ Low Input cDNA Synthesis & Amplification Kit?

The kit can work with RNA inputs as low as 10 pg or single cells.

How long does the cDNA synthesis and amplification process take with this kit?

The entire process takes approximately 5 hours.

Is this kit compatible with RNA from different sources?

Yes, the kit is compatible with RNA from a variety of sources, including mammalian, plant, and microbial cells.

What downstream applications can be performed with the cDNA generated using this kit?

The amplified cDNA is suitable for a range of downstream applications, including sequencing, gene expression profiling, and microarray analysis.

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