The SeqVersa™ Fragmented FFPE DNA Library Prep Kit for Illumina offers a comprehensive solution for high-quality DNA fragmentation and library preparation. Utilizing a proprietary enzymatic shearing technology, this kit ensures precise and consistent fragment sizes, making it ideal for next-generation sequencing applications.
SeqVersa™ Fragmented FFPE DNA Library Prep Kit
Price range: $860.00 through $3,270.00
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The SeqVersa™ Fragmented FFPE DNA Library Prep Kit for Illumina is designed to streamline the process of DNA library preparation with an emphasis on efficiency and reliability. This kit employs a novel enzymatic shearing method that delivers uniform DNA fragmentation, which is crucial for the accuracy and consistency of next-generation sequencing (NGS) data. By replacing traditional mechanical shearing techniques, UltraShear™ reduces variability and simplifies the workflow, ensuring high-quality libraries with minimal hands-on time.
One of the standout features of the SeqVersa™ Fragmented FFPE DNA Library Prep Kit is its flexibility. It is compatible with a wide range of DNA input amounts, from low nanogram to microgram quantities, making it suitable for various sample types and research applications. The kit’s components are meticulously optimized to work together seamlessly, providing superior end-repair and ligation efficiency. Additionally, the included adapters and PCR reagents are specifically formulated to minimize bias and enhance the uniformity of coverage across different genomic regions.
The SeqVersa™ Fragmented FFPE DNA Library Prep Kit also includes thorough quality control steps to ensure that only the highest-quality libraries proceed to sequencing. This includes size selection and cleanup steps using magnetic beads, which are critical for removing unwanted fragments and contaminants. The kit is designed for ease of use, with a streamlined protocol that reduces the risk of errors and improves reproducibility.
Overall, the SeqVersa™ Fragmented FFPE DNA Library Prep Kit for Illumina offers researchers a reliable and efficient solution for DNA library preparation, enabling them to generate high-quality sequencing data with confidence. Whether for whole genome sequencing, targeted sequencing, or other genomic applications, this kit provides the tools needed to achieve exceptional results.
Kit Contents
● Enzymatic Fragmentation Mix
● End Repair Reaction Buffer
● End Repair Enzyme Mix
● Adapter Ligation Reaction Buffer
● Adapter Ligation Enzyme Mix
● PCR Master Mix
● DNA Size Selection Beads
● DNA Cleanup Beads
Technical Parameters
● Input DNA Amount: 1 ng – 1 µg
● Fragmentation Time: 10-30 minutes
● Library Yield: High yield with low bias
● Compatibility: Compatible with Illumina sequencing platforms
● Storage Conditions: -20°C for all reagents
Applications
● Whole Genome Sequencing (WGS)
● Targeted Sequencing
● Metagenomics
● Epigenomics
● Transcriptomics
Product FAQs
What is the minimum DNA input required for the SeqVersa™ Fragmented FFPE DNA Library Prep Kit?
The kit can accommodate DNA input amounts as low as 1 ng, making it suitable for samples with limited availability.
How does the enzymatic shearing method compare to mechanical shearing?
Enzymatic shearing offers more precise and consistent fragment sizes, reduces variability, and simplifies the workflow compared to traditional mechanical shearing methods.
Is the SeqVersa™ Fragmented FFPE DNA Library Prep Kit compatible with all Illumina sequencing platforms?
Yes, the kit is designed to be fully compatible with all Illumina sequencing platforms, ensuring versatility for various NGS applications.
Can this kit be used for both high and low-GC-content genomes?
Yes, the kit is optimized to provide uniform coverage across genomes with varying GC content, minimizing bias in the sequencing data.
How do I ensure optimal fragment size distribution using this kit?
Follow the recommended shearing times and conditions provided in the user manual. Additionally, performing a test run to optimize conditions for your specific sample type can help achieve the desired fragment size distribution.
What are the storage conditions for the reagents in this kit?
All reagents should be stored at -20°C to ensure their stability and performance over time.
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