Price range: $178.00 through $616.00
The SeqVersa™ FFPE DNA Repair Kit is a highly efficient enzyme mix designed to repair damaged DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples, ensuring high-quality DNA for downstream applications like next-generation sequencing (NGS).
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The SeqVersa™ FFPE DNA Repair Kit is an innovative solution tailored to address the unique challenges posed by DNA extracted from formalin-fixed, paraffin-embedded (FFPE) tissue samples. FFPE samples are commonly used in clinical and research settings due to their ability to preserve cellular architecture and protein integrity. However, the fixation process can cause significant DNA damage, including fragmentation, cross-linking, and base modifications, which can hinder accurate and efficient downstream applications such as next-generation sequencing (NGS).
This repair mix includes a combination of highly specific enzymes that work synergistically to repair various types of DNA damage. These enzymes include endonucleases, exonucleases, DNA polymerases, and ligases, each playing a critical role in recognizing and repairing nicks, gaps, and other forms of DNA damage. The use of the SeqVersa™ FFPE DNA Repair Kit results in higher integrity DNA, making it suitable for high-quality library preparation and reliable sequencing.
The repair process is straightforward and can be seamlessly integrated into existing workflows. After the extraction of DNA from FFPE samples, the repair mix is added, and the reaction is incubated under optimized conditions. The repaired DNA is then purified, making it ready for subsequent library preparation and sequencing.
Applications, including whole genome sequencing, targeted sequencing, and epigenetic studies. By improving the quality of FFPE-derived DNA, the SeqVersa™ FFPE DNA Repair Kit enhances the accuracy and reliability of sequencing data, which is critical for applications in cancer research, pathology, and biomarker discovery.
● FFPE DNA Repair Enzyme Mix
● FFPE DNA Repair Buffer
● DNA Cleanup Beads
● Input DNA Amount: 10 ng – 1 µg
● Repair Efficiency: High efficiency with low bias
● Compatibility: Compatible with various downstream NGS applications
● Storage Conditions: -20°C for all reagents
● Cancer Genomics
● Pathology Research
● Biomarker Discovery
● Epigenetic Studies
● Clinical Research
The kit can accommodate DNA input amounts as low as 10 ng, making it suitable for samples with limited availability.
The repair mix includes specific enzymes that recognize and repair various types of DNA damage, such as nicks, gaps, and base modifications, resulting in higher-integrity DNA suitable for sequencing.
Yes, the repaired DNA can be used with various NGS platforms, including Illumina, making it versatile for different sequencing applications.
Yes, the mix is designed to repair highly degraded DNA from FFPE samples, improving the quality and yield of DNA for downstream applications.
All reagents should be stored at -20°C to ensure their stability and performance over time.
The entire repair process, including incubation, can be completed in approximately 1-2 hours, depending on the specific protocol used.
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