The SeqVersa™ FFPE DNA Library Prep Kit provides a streamlined and efficient workflow for preparing high-quality DNA libraries from formalin-fixed, paraffin-embedded (FFPE) tissue samples, optimized for next-generation sequencing (NGS) applications.
SeqVersa™ FFPE DNA Library Prep Kit
Price range: $754.00 through $2,870.00
Purchase this item and get Product Description
The SeqVersa™ FFPE DNA Library Prep Kit is meticulously designed to meet the unique challenges associated with processing formalin-fixed, paraffin-embedded (FFPE) tissue samples for next-generation sequencing (NGS). FFPE samples are widely used in clinical and research settings due to their ability to preserve tissue morphology and molecular integrity. However, the fixation process often leads to DNA fragmentation and chemical modifications that complicate downstream applications. This library prep kit addresses these issues through a series of optimized enzymatic reactions that repair and prepare FFPE-derived DNA for high-quality sequencing.
The kit includes reagents for DNA repair, end preparation, adapter ligation, and PCR enrichment, offering a comprehensive solution for library preparation. The DNA repair step is critical for restoring damaged DNA, ensuring higher yield and quality of the final library. This is followed by end repair and dA-tailing, which prepare the DNA fragments for adapter ligation. The inclusion of high-efficiency ligation reagents ensures robust and consistent attachment of sequencing adapters, while the subsequent PCR amplification step enriches the library, making it ready for sequencing.
The SeqVersa™ FFPE DNA Library Prep Kit is compatible with a broad range of DNA input amounts, from as little as 50 ng to 1 µg. providing flexibility for various sample types and research needs. The kit’s streamlined workflow minimizes hands-on time and reduces the potential for sample loss and contamination. Magnetic bead-based purification steps are included to facilitate efficient cleanup and size selection, ensuring that only the highest quality DNA fragments are retained for sequencing.
By enabling the reliable preparation of high-quality DNA libraries from challenging FFPE samples, the SeqVersa™ FFPE DNA Library Prep Kit empowers researchers to generate accurate and reproducible NGS data. This kit is particularly valuable for applications in oncology, pathology, and clinical research, where FFPE samples are commonly used.
Kit Contents
● FFPE DNA Repair Mix
● End Repair Reaction Buffer
● End Repair Enzyme Mix
● Adapter Ligation Reaction Buffer
● Adapter Ligation Enzyme Mix
● PCR Master Mix
● DNA Size Selection Beads
● DNA Cleanup Beads
Technical Parameters
● Input DNA Amount: 50 ng – 1 µg
● Library Yield: High yield with low bias
● Compatibility: Compatible with Illumina sequencing platforms
● Storage Conditions: -20°C for all reagents
Applications
● Cancer Research
● Pathology Studies
● Clinical Research
● Biomarker Discovery
● Epigenetics
Product FAQs
What is the minimum DNA input required for the SeqVersa™ FFPE DNA Library Prep Kit?
The kit can accommodate DNA input amounts as low as 50 ng, making it suitable for samples with limited availability.
How does the DNA repair step benefit FFPE samples?
The DNA repair step restores fragmented and chemically modified DNA, improving the quality and yield of the final library, which is crucial for accurate sequencing results.
Is the SeqVersa™ FFPE DNA Library Prep Kit compatible with all Illumina sequencing platforms?
Yes, the kit is designed to be fully compatible with all Illumina sequencing platforms, ensuring versatility for various NGS applications.
Can this kit handle highly degraded DNA from older FFPE samples?
Yes, the kit includes specialized reagents to repair and prepare highly degraded DNA, making it suitable for use with older FFPE samples.
What are the storage conditions for the reagents in this kit?
All reagents should be stored at -20°C to ensure their stability and performance over time
How long does the library preparation process take?
The entire library preparation process, including DNA repair, end repair, adapter ligation, and cleanup, can be completed in approximately 4-5 hours, depending on the sample type and the specific protocol used.
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