SeqVersa™ End Repair Kit facilitates efficient end repair and dA-tailing of DNA fragments, essential for preparing libraries compatible with Illumina sequencing platforms.
SeqVersa™ End Repair Kit
Price range: $285.00 through $909.00
Purchase this item and get Product Description
SeqVersa™ End Repair Kit is a comprehensive solution designed for precise and efficient end repair and dA-tailing of DNA fragments in preparation for library construction. This kit ensures high-quality library generation compatible with Illumina sequencing platforms, enhancing the accuracy and reliability of sequencing results.
The end repair process is crucial in library preparation workflows, where it repairs DNA ends by removing damaged bases and creating blunt ends suitable for subsequent adapter ligation. The addition of dA-tails allows for efficient adapter ligation, increasing the library complexity and sequencing efficiency.
This kit includes optimized reagents and buffers for streamlined and robust end repair and dA-tailing reactions. The protocol is user-friendly, requiring minimal hands-on time and compatible with a wide range of input DNA amounts and qualities. SeqVersa™ End Repair Kit is suitable for various applications, including whole-genome sequencing, targeted sequencing, and transcriptome analysis.
Key features of the SeqVersa™ End Repair Kit include high efficiency, low background noise, and consistent performance across different DNA sources and sizes. It ensures uniform library representation, enabling researchers to obtain accurate and reproducible sequencing data.
Kit Contents
● SeqVersa™ End Repair Enzyme Mix
● SeqVersa™ dA-Tailing Buffer
● SeqVersa™ ATP Solution
Technical Parameters
● Input DNA Amount: 100 pg to 1 μg
● Compatibility: Suitable for Illumina sequencing platforms
● Library Complexity: High complexity with reduced bias
● Storage Conditions: -20°C for all components
● Protocol Time: Approximately 1.5 hours
Applications
● Whole-genome sequencing
● Targeted sequencing
● ChIP-Seq
● Transcriptome analysis
● DNA repair studies
Product FAQs
What is the purpose of the SeqVersa™ End Repair Kit?
The kit is used for efficient end repair and dA-tailing of DNA fragments, essential for preparing libraries compatible with Illumina sequencing platforms.
What is the input DNA amount required for using the SeqVersa™ End Repair Kit?
The kit is optimized for input DNA amounts ranging from 100 pg to 1 μg.
Is SeqVersa™ End Repair Kit compatible with Illumina sequencing platforms?
Yes, SeqVersa™ End Repair Kit is fully compatible with Illumina sequencing platforms.
How long does the end repair and dA-tailing process take?
The entire process takes approximately 1.5 hours, including minimal hands-on time.
What are the advantages of using the SeqVersa™ End Repair Kit?
SeqVersa™ End Repair Kit offers high efficiency, low background noise, and consistent performance for preparing high-quality DNA libraries.
| Reactions | 24 reactions, 96 reactions |
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