SeqVersa™ dsDNA Fragmentase provides a rapid and efficient method for generating randomly fragmented DNA, ideal for next-generation sequencing (NGS) library preparation and other genomic applications.
SeqVersa™ dsDNA Fragmentase
Price range: $120.00 through $479.00
Purchase this item and get Product Description
The SeqVersa™ dsDNA Fragmentase is a highly efficient enzymatic system designed for the controlled fragmentation of double-stranded DNA (dsDNA). This kit is tailored to meet the needs of researchers preparing samples for next-generation sequencing (NGS) and other genomic analyses that require uniform and random DNA fragments.
At the heart of the SeqVersa™ dsDNA Fragmentase kit is a proprietary blend of enzymes that ensures consistent and reproducible fragmentation. The kit allows for precise control over fragment size by adjusting reaction conditions, making it suitable for a variety of applications that demand specific DNA fragment lengths. The enzymatic approach offers significant advantages over physical shearing methods, such as sonication, by minimizing sample loss and maintaining the integrity of DNA ends, which is crucial for subsequent library construction.
This kit is designed for ease of use, with a straightforward protocol that integrates seamlessly into existing workflows. Researchers can achieve high yields of uniformly fragmented DNA with minimal hands-on time, enhancing the efficiency of their experiments. The fragmented DNA produced is ideal for use in a range of applications, including whole-genome sequencing, targeted sequencing, and other NGS workflows.
SeqVersa™ dsDNA Fragmentase is compatible with various types of dsDNA, including genomic DNA from different organisms, plasmid DNA, and PCR products. Its versatility and reliability make it an essential tool for modern genomic research, providing consistent and high-quality results every time.
Kit Contents
● Fragmentase Enzyme Mix
● Reaction Buffer
● Nuclease-Free Water
● Stop Solution
Technical Parameters
● Input DNA Quantity: 100 ng to 1 µg
● Fragment Size Range: Adjustable from 100 bp to 1 kb
● Reaction Time: 5 to 30 minutes, depending on desired fragment size
● Storage Conditions: -20°C for all reagents
● Compatibility: Suitable for genomic DNA, plasmid DNA, and PCR
products
Applications
● Next-Generation Sequencing (NGS) library preparation
● Whole-genome sequencing
● Targeted sequencing
● Fragmentation for hybrid capture and enrichment workflows
● Any application requiring uniform and random DNA fragmentation
Product FAQs
What is the typical input DNA amount required for the SeqVersa™ dsDNA Fragmentase kit?
The kit is designed to work with 100 ng to 1 µg of input dsDNA.
How can I control the size of the DNA fragments produced?
Fragment size can be adjusted by varying the reaction time and enzyme concentration. The protocol provides detailed guidelines for achieving different fragment sizes.
Is the SeqVersa™ dsDNA Fragmentase compatible with DNA from different sources?
Yes, it is compatible with genomic DNA, plasmid DNA, and PCR products from various organisms.
What is the typical reaction time for DNA fragmentation using this kit?
The reaction time ranges from 5 to 30 minutes, depending on the desired fragment size. Detailed instructions are provided in the user guide.
| Reactions | 250, 50 |
|---|
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