SeqVersa™ Taq DNA Polymerase is a thermostable enzyme ideal for high-specificity PCR amplification. It features an antibody-mediated hot-start mechanism, reducing non-specific amplification and provides robust performance across a variety of templates.
SeqVersa™ DNA Polymerase
Price range: $75.00 through $350.00
Purchase this item and get Product Description
SeqVersa™ Taq DNA Polymerase is a high-fidelity enzyme designed to enhance the specificity and efficiency of PCR amplification. The enzyme is activated at high temperatures, preventing non-specific amplification and primer-dimer formation by using an antibody-mediated hot-start mechanism. This makes it particularly useful for complex templates or those with high GC content. The hot start capability ensures that the enzyme remains inactive at lower temperatures, providing a clear advantage over traditional Taq DNA polymerases by reducing non-specific binding. The enzyme’s high performance across various templates, combined with its robust amplification capabilities, makes it an excellent choice for both routine and challenging PCR applications. SeqVersa™ Taq DNA Polymerase is suitable for amplifying DNA for cloning, sequencing, and analysis, ensuring reliable results with minimal background. Its compatibility with a wide range of reaction conditions and target sequences makes it versatile for various research needs. The polymerase has an optimized buffer system, ensuring consistent performance and ease of use in different PCR protocols.
Kit Contents
● SeqVersa™ Taq DNA Polymerase (100 U)
● 10X SeqVersa™ Reaction Buffer (1.25 mL)
● 25 mM MgCl2 (1.5 mL)
● 10 mM dNTP Mix (0.2 mL)
Technical Parameters
● Enzyme Concentration: 5 U/µl
● Activation: Antibody-mediated hot start
● Storage: -20°C
● Optimal Extension Temperature: 72°C
● Half-life: >40 min at 95°C
Applications
● High-specificity PCR
● DNA cloning
● DNA sequencing
● Mutagenesis
● Genotyping
Product FAQs
What is the advantage of hot-start Taq DNA polymerase?
The hot start mechanism prevents non-specific amplification and primer-dimer formation, increasing the specificity and yield of PCR products.
How should I store the SeqVersa™ Taq DNA Polymerase?
Store the enzyme at -20°C to maintain its stability and activity.
Can I use this enzyme for high GC content templates?
Yes, SeqVersa™ Taq DNA Polymerase is designed to perform well with high GC content templates.
What is the optimal extension temperature for this polymerase?
The optimal extension temperature for SeqVersa™ Taq DNA Polymerase is 72°C.
| Reactions | 100, 500 |
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