SeqVersa™ Directional Second Strand Synthesis Kit enables efficient synthesis of strand-specific cDNA for RNA sequencing, ensuring accurate and reproducible results.
SeqVersa™ Directional Second Strand Synthesis Kit
Price range: $441.00 through $1,407.00
Purchase this item and get Product Description
SeqVersa™ Directional Second Strand Synthesis Kit is specifically designed for the synthesis of strand-specific cDNA during RNA library preparation for Illumina sequencing. This kit ensures precise orientation information of RNA transcripts, crucial for applications such as differential gene expression analysis, RNA isoform identification, and non-coding RNA characterization.
The kit includes optimized reagents and protocols for robust second-strand cDNA synthesis, incorporating dUTP during the process to mark the second strand. This enables the selective removal of the second strand during library construction, ensuring strand-specific sequencing. The protocol maintains high fidelity and efficiency, allowing researchers to obtain an accurate representation of RNA transcripts with minimal bias.
Key components of the SeqVersa™ Directional Second Strand Synthesis Kit include buffers, enzymes, and sample purification beads. These components are rigorously tested for performance consistency and are suitable for a wide range of RNA input amounts and sample types, including total RNA and mRNA from various biological sources.
Kit Contents
● Second Strand Synthesis Enzyme Mix
● dUTP Solution
● Second Strand Synthesis Buffer
● Sample Purification Beads
Technical Parameters
● Input RNA Amount: 10 ng to 1 μg
● Library Type: Strand-specific cDNA libraries
● Compatibility: Illumina sequencing platforms
● Protocol Time: Approximately 3-4 hours
Applications
● Differential gene expression analysis
● RNA isoform identification
● Non-coding RNA characterization
● Long non-coding RNA (lncRNA) analysis
Product FAQs
What is the purpose of the SeqVersa™ Directional Second Strand Synthesis Kit?
The kit is designed for the synthesis of strand-specific cDNA during RNA library preparation, essential for accurate RNA sequencing and analysis.
What RNA input amounts are suitable for the SeqVersa™ Directional Second Strand Synthesis Kit?
The kit is optimized for input RNA amounts ranging from 10 ng to 1 μg.
Is the SeqVersa™ Directional Second Strand Synthesis Kit compatible with Illumina sequencing platforms?
Yes, the kit is compatible with Illumina sequencing platforms, ensuring high-quality RNA sequencing data.
How does the kit ensure strand-specific sequencing?
The kit incorporates dUTP during the second strand synthesis, allowing selective removal of the second strand during library construction, thereby ensuring strand-specific sequencing.
What are the advantages of using the SeqVersa™ Directional Second Strand Synthesis Kit?
SeqVersa™ Directional Second Strand Synthesis Kit offers high fidelity, efficiency, and reproducibility, providing accurate representation of RNA transcripts in RNA sequencing experiments.
| Reactions | 24 reactions, 96 reactions |
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