SeqVersa™ Directional RNA Library Prep Kit enables robust and efficient preparation of directional RNA libraries for Illumina sequencing, ensuring accurate strand-specific RNA analysis.
SeqVersa™ Directional RNA Library Prep Kit
Price range: $1,081.00 through $3,672.00
Purchase this item and get Product Description
SeqVersa™ Directional RNA Library Prep Kit is designed to streamline the preparation of strand-specific RNA libraries compatible with Illumina sequencing platforms. This kit facilitates the conversion of RNA into cDNA libraries with precise orientation information, essential for applications such as differential gene expression analysis, RNA splicing characterization, and small RNA discovery.
Key components of the SeqVersa™ Directional RNA Library Prep Kit include enzymatic modules for RNA fragmentation, first and second strand cDNA synthesis, end repair, adapter ligation, and PCR amplification. The protocol is optimized to maintain RNA strand specificity throughout the library preparation process, minimizing bias and ensuring accurate representation of RNA transcripts.
The kit includes reagents and buffers that are rigorously tested for performance consistency, ensuring reproducible results across different RNA inputs and sample types. It supports a wide range of starting RNA amounts and is compatible with various RNA sources, including total RNA and mRNA from diverse biological samples.
Kit Contents
● RNA Fragmentation Module
● First Strand Synthesis Module
● Second Strand Synthesis Module
● End Repair Module
● Adapter Ligation Module
● PCR Master Mix
Technical Parameters
● Input RNA Amount: 10 ng to 1 μg
● Library Type: Strand-specific RNA libraries
● Compatibility: Illumina sequencing platforms
● Protocol Time: Approximately 4-5 hours
Applications
● Differential gene expression analysis
● RNA splicing characterization
● Small RNA discovery
● Non-coding RNA analysis
Product FAQs
What is the advantage of using SeqVersa™ Directional RNA Library Prep Kit?
The kit enables the preparation of strand-specific RNA libraries, crucial for accurate RNA analysis and interpretation.
What RNA input amounts are suitable for SeqVersa™ Directional RNA Library Prep Kit?
The kit is optimized for input RNA amounts ranging from 10 ng to 1 μg.
Is the SeqVersa™ Directional RNA Library Prep Kit compatible with Illumina sequencing platforms?
Yes, the kit is designed for compatibility with Illumina sequencing platforms.
How long does the library preparation process take with SeqVersa™ Directional RNA Library Prep Kit?
The process typically takes approximately 4-5 hours, depending on the specific protocol and RNA input amount.
What types of RNA can be used with SeqVersa™ Directional RNA Library Prep Kit?
The kit supports various RNA sources, including total RNA and mRNA, from different biological samples.
| Reactions | 24 reactions, 96 reactions |
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