SeqVersa™ Bacterial RNA Depletion

Price range: $281.00 through $3,688.00

SeqVersa™ Bacterial RNA Depletion Kit provides comprehensive and efficient removal of rRNA and other unwanted RNA species from total RNA samples, enhancing the quality of RNA-Seq data.

Purchase this item and get 28-368 Points
Purchase this item and get 28-368 Points
Product Description

SeqVersa™ Bacterial RNA Depletion Kit is a powerful and efficient solution designed to enhance the quality and accuracy of RNA sequencing (RNA-Seq) data by selectively depleting ribosomal RNA (rRNA) and other unwanted RNA species from total RNA samples. This reagent set is
optimized to improve the representation of coding and non-coding RNA by reducing the dominance of rRNA, which constitutes the majority of total RNA. The SeqVersa™ Bacterial RNA Depletion Kit employs a targeted hybridization approach, which ensures high specificity and efficiency in the removal of rRNA from a wide variety of sample types, including human, mouse, and rat RNA. This method significantly reduces background noise, resulting in higher sensitivity and more meaningful sequencing data.

This reagent set is designed for ease of use and compatibility with downstream RNA-Seq workflows. The protocol is straightforward, with minimal hands-on time and no need for additional equipment, making it suitable for high-throughput sequencing applications. By effectively depleting rRNA, SeqVersa™ Bacterial RNA Depletion Kit allows for deeper sequencing of the transcriptome, providing more comprehensive insights into gene expression, regulatory mechanisms, and RNA modifications.

Key benefits of using SeqVersa™ Bacterial RNA Depletion Kit include improved data quality, increased sequencing efficiency, and enhanced detection of low-abundance transcripts. These features make it an ideal choice for researchers seeking to maximize the utility of their RNA-Seq experiments. 

Kit Contents

●  SeqVersa™ rRNA Depletion Reagent
●  SeqVersa™ Hybridization Buffer
●  SeqVersa™ Beads for rRNA Capture
●  SeqVersa™ Elution Buffer

Technical Parameters

●  Depletion Efficiency: >90% rRNA depletion
●  Sample Types: Human, mouse, rat RNA
●  Compatibility: Compatible with a variety of RNA-Seq library preparation methods
●  Input RNA Amount: 10 ng to 1 μg of total RNA
●  Storage Conditions: -20°C for all components
●  Protocol Time: Approximately 3 hours

Applications

●  RNA-Seq library preparation
●  Transcriptome analysis
●  Gene expression profiling
●  Non-coding RNA studies
●  RNA modification studies

Product FAQs
What is the purpose of the SeqVersa™ Bacterial RNA Depletion Kit?

The reagent set is used to remove ribosomal RNA and other unwanted RNA species from total RNA samples, enhancing the quality and accuracy of RNA-Seq data.

What types of RNA samples are compatible with SeqVersa™ Bacterial RNA Depletion Kit?

The reagent set is compatible with RNA samples from humans, mice, and rats, and can be used with various RNA-Seq library preparation methods.

How efficient is the rRNA depletion with SeqVersa™ Bacterial RNA Depletion Kit?

The reagent set achieves greater than 90% depletion of rRNA, significantly reducing background noise in RNA-Seq data.

What is the input RNA amount required for using SeqVersa™ Bacterial RNA Depletion Kit?

The protocol is optimized for input amounts ranging from 10 ng to 1 μg of total RNA.

How long does the depletion process take?

The entire depletion protocol takes approximately 3 hours, including minimal hands-on time.

Reactions

24 reactions, 6 reactions, 96 reactions

SKU: RD3917 Category:
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