The SeqVersa™ 5hmC Sequencing Kit provides a comprehensive solution for identifying and analyzing 5-hydroxymethylcytosine (5hmC) modifications in DNA. This kit is optimized for high sensitivity and accuracy, facilitating advanced epigenetic research.
SeqVersa™ 5hmC Sequencing Kit
$533.00
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The SeqVersa™ 5hmC Sequencing Kit is an innovative tool designed to enable the precise detection and analysis of 5-hydroxymethylcytosine (5hmC) modifications in genomic DNA. 5hmC is a crucial epigenetic marker involved in various biological processes, including gene regulation, development, and disease pathology. Understanding its distribution and dynamics can provide deep insights into epigenetic regulation mechanisms. This kit utilizes an enzymatic approach for the selective conversion and sequencing of 5hmC residues, ensuring high specificity and efficiency. The included enzymes and buffers are meticulously optimized to achieve reliable conversion without compromising the integrity of the DNA, allowing for accurate downstream sequencing and analysis.
SeqVersa™ 5hmC Sequencing Kit’s protocol integrates seamlessly with next-generation sequencing (NGS) workflows, providing a straightforward and efficient method for researchers. The kit is compatible with various NGS platforms, enabling flexibility in experimental design and application. Whether you are studying 5hmC distribution across the genome, investigating its role in gene regulation, or exploring its potential as a biomarker, the SeqVersa™ 5hmC Sequencing Kit offers a robust and reliable solution.
Kit Contents
● T4 β-Glucosyltransferase (T4-BGT)
● UDP-Glucose
● Oxidation Reagent
● Conversion Buffer
● Cleanup Reagents
Technical Parameters
● Input DNA Quantity: 100 ng – 1 µg
● Reaction Volume: 50 µl
● Conversion Time: 2 hours
● Storage Conditions: -20°C
● Compatibility: Compatible with various NGS platforms (e.g., Illumina)
Applications
● Detection and analysis of 5-hydroxymethylcytosine (5hmC)
● Epigenetic research and profiling
● Biomarker discovery and validation
● Understanding gene regulation mechanisms
● Studying disease-related epigenetic changes
Product FAQs
What is the minimum amount of DNA required for the SeqVersa™ 5hmC Sequencing Kit?
The kit requires a minimum of 100 ng of input DNA to ensure efficient conversion and accurate detection of 5hmC modifications.
Can this kit be used with different next-generation sequencing platforms?
Yes, the SeqVersa™ 5hmC Sequencing Kit is compatible with various NGS platforms, including Illumina and Oxford Nanopore, providing flexibility for different experimental setups.
How specific is the conversion process for 5hmC using this kit?
The kit employs a highly specific enzymatic conversion process that selectively targets 5hmC, ensuring minimal background and high accuracy in detecting 5hmC modifications.
What types of studies can benefit from using the SeqVersa™ 5hmC Sequencing Kit?
This kit is ideal for a wide range of epigenetic studies, including gene regulation research, biomarker discovery, disease epigenetics, and comprehensive 5hmC profiling across different biological samples.
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