SeqVersa™ 5hmC-seq Conversion Kit

SeqVersa™ 5hmC-seq Conversion Kit

Price range: $119.00 through $451.00

The SeqVersa™ 5hmC-seq Conversion Kit provides a reliable and efficient method for converting 5-hydroxymethylcytosine (5hmC) in DNA, enabling accurate detection and analysis of epigenetic modifications through next-generation sequencing.

Purchase this item and get 11-45 Points
Purchase this item and get 11-45 Points
Product Description

The SeqVersa™ 5hmC-seq Conversion Kit is a cutting-edge solution for researchers looking to explore the epigenetic landscape of DNA. Traditional bisulfite sequencing methods for methylation analysis often result in DNA degradation and incomplete conversion of 5hmC to uracil, leading to inaccurate data. This module addresses these issues by utilizing an enzymatic approach that specifically converts 5hmC to 5-formylcytosine (5fC), which is subsequently converted to uracil. This conversion method preserves DNA integrity and provides a more accurate representation of methylation and hydroxymethylation status.

This module is particularly useful for studying the role of 5hmC in gene regulation, development, and disease. The enzymatic conversion process is gentle on DNA, ensuring higher yields and better-quality libraries for sequencing. Additionally, the protocol is streamlined and compatible with standard library preparation workflows, making it easy to integrate into existing sequencing projects

The SeqVersa™ 5hmC-seq Conversion Kit is designed for use with a wide range of input DNA amounts, providing flexibility for various experimental needs. The kit includes all necessary reagents for the conversion process, along with a detailed protocol to guide users through each step. By offering a reliable and efficient method for detecting 5hmC, this module enables researchers to gain deeper insights into epigenetic modifications and their implications in health and disease.

Kit Contents

● TET2 Enzyme Mix
● Oxidation Reaction Buffer
● Glucosyltransferase Enzyme Mix
● Glucosylation Reaction Buffer
● Uracil DNA Glycosylase (UDG) Enzyme Mix
● UDG Reaction Buffer
● DNA Cleanup Beads

Technical Parameters​

● Input DNA Amount: 10 ng – 1 µg
● Conversion Efficiency: > 99%
● Library Yield: High yield with low degradation
● Compatibility: Compatible with Illumina sequencing platforms
● Storage Conditions: -20°C for all reagents

Applications

● Methylation Analysis
● Hydroxymethylation Analysis
● Epigenomics
● Cancer Research
● Developmental Biology

Product FAQs
What is the minimum DNA input required for the SeqVersa™ 5hmC-seq Conversion Kit?

The kit can accommodate DNA input amounts as low as 10 ng, making it suitable for samples with limited availability.

How does the enzymatic conversion method compare to traditional bisulfite sequencing?

The enzymatic method is gentler on DNA, resulting in higher yields and better-quality libraries. It also provides a more accurate conversion of 5hmC to uracil, improving the reliability of methylation and hydroxymethylation data.

Is the SeqVersa™ 5hmC-seq Conversion Kit compatible with all Illumina sequencing platforms?

Yes, the module is designed to be fully compatible with all Illumina sequencing platforms, ensuring versatility for various NGS applications.

Can this kit be used for both high and low-GC-content genomes?

Yes, the kit is optimized to provide uniform coverage across genomes with varying GC content, minimizing bias in the sequencing data.

What are the storage conditions for the reagents in this kit?

All reagents should be stored at -20°C to ensure their stability and performance over time.

How long does the conversion process take?

The entire conversion process, including oxidation and glucosylation steps, can be completed in approximately 4-5 hours, depending on the sample type and the specific protocol used.

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