The RevDNA™ Plasmid Kits facilitates fast and efficient large-scale plasmid DNA preparation without centrifugation. With features like lysate clearing and isopropanol precipitation without the need for a vacuum manifold, it offers up to 750 μg yield of high-copy plasmid DNA in less than 60 minutes.
RevDNA™ Plasmid Kits
Price range: $406.00 through $990.00
Purchase this item and get Product Description
The RevDNA™ Plasmid Kits streamline the process of large-scale plasmid DNA preparation, offering convenience and efficiency. It eliminates the need for centrifugation during lysate clearing and isopropanol precipitation, reducing processing time to less than 60 minutes. The purified DNA obtained from this kit is equivalent to that obtained by 2 x CsCl gradient centrifugation, making it suitable for a wide range of molecular biology applications, including transfection, cloning, sequencing, and gene silencing. The unique design of RevDNA™ Tips and Precipitator modules ensures high yields of pure DNA with no risk of pellet loss, providing researchers with reliable results every time.
Kit Contents
● RevDNA™ Tips
● Filter Cartridges
● Precipitator Modules
● Reagents
● Buffers
● Syringes
Technical Parameters
● Scalable purification from 150 μl to 250 ml culture volume (Maxi Kit) or 50 μl to 150 ml culture volume (Midi Kit)
● Processing of 1 sample per run
● Time per run: 60 minutes (Maxi Kit) or 45 minutes (Midi Kit)
● Yield: Up to 750 μg (Maxi Kit) or 200 μg (Midi Kit) of high-copy plasmid DNA
Applications
● Transfection
● Cloning
● Sequencing
● Gene silencing
Product FAQs
Can the RevDNA™ Plasmid Kits be used for other types of DNA purification?
No, this kit is specifically designed for large-scale plasmid DNA preparation and is not recommended for other types of DNA purification.
Is the RevDNA™ Plasmid Kits suitable for high-throughput applications?
Yes, this kit offers fast processing and high yields of plasmid DNA, making it suitable for high-throughput applications requiring large-scale DNA purification.
Can the purified DNA be stored for future use?
Yes, the purified DNA can be stored at appropriate temperatures (-20°C) for long-term use in various downstream applications without compromising its quality and integrity.
| Kit type | Midi, Maxi |
|---|---|
| Reactions | 10, 25 |
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