Achieve fast and efficient large-scale plasmid DNA purification with the RevDNA™ Mega Plasmid Kits, delivering up to 10 mg of high-copy DNA yield without the need for centrifugation.
RevDNA™ Mega Plasmid Kits
Price range: $672.00 through $1,146.00
Purchase this item and get Product Description
The RevDNA™ Mega Plasmid Kits revolutionize large-scale plasmid DNA purification by combining speed, efficiency, and reliability. Designed for molecular biology applications, this kit empowers researchers to obtain ultra-pure DNA with unparalleled convenience and ease of use. With a streamlined workflow and innovative features, including vacuum-driven purification and integrated endotoxin removal, the RevDNA™ Mega Plasmid Kits sets a new standard for high-throughput DNA preparation. By leveraging the power of anion-exchange technology, the kit enables rapid purification of high-copy plasmid DNA from large culture volumes without the need for time-consuming centrifugation steps. The Filter Cartridges efficiently remove cellular debris and contaminants, while the RevDNA™ Tips facilitate high-flow-rate DNA binding, washing, and elution, ensuring maximum yield and purity.
Additionally, the Concentrator modules simplify DNA concentration and desalting, further streamlining the purification process. One of the key advantages of the RevDNA™ Mega Plasmid Kits is its ability to deliver endotoxin-free DNA, essential for sensitive applications such as transfection and gene therapy research. The integrated endotoxin removal step ensures that the purified DNA meets the stringent quality requirements for reproducible and reliable results. This makes the kit an invaluable tool for researchers working with primary cells, sensitive cultured cells, and other advanced applications where purity is paramount With a processing time of just 50 minutes per run, the RevDNA™ Mega Plasmid Kits offer unmatched efficiency, allowing laboratories to achieve high-throughput DNA purification with minimal hands-on time. Whether performing cloning, sequencing, transfection, or gene silencing experiments, researchers can trust the RevDNA™ Mega Plasmid Kits to deliver consistent and high-quality results, accelerating their scientific discoveries In summary, the RevDNA™ Mega Plasmid Kits represent a significant advancement in large-scale plasmid DNA purification technology. Combining speed, efficiency, and reliability, this kit empowers researchers to streamline their workflows, save time, and obtain ultra-pure DNA suitable for a wide range of molecular biology applications.
Kit Contents
● Vacuum manifold
● Filter Cartridges
● RevDNA™ Tips
● Concentrator modules
● Endotoxin Removal Buffer
Technical Parameters
● Applications: Transfection, cloning sequencing, gene silencing
● Culture volume/starting material: 500 ml culture volume for Mega EF
Kit, 2.5 l culture volume for Giga EF Kit
● Plasmid type: High-copy, cosmid DNA
● Processing: Vacuum, centrifugation
● Sample per run: 6 samples per run
● Technology: Anion-exchange technology
● Time per run: 50 minutes
● Yield: 2.5 mg for Mega EF Kit, 10 mg for Giga EF Kit
Applications
For applications, including cloning, sequencing, transfection of sensitive
cells, primary cells, and gene silencing.
Product FAQs
What is the advantage of using the RevDNA™ Mega Plasmid Kits?
These kits offer fast and efficient large-scale plasmid DNA purification, delivering high yields of DNA without the need for centrifugation, making them ideal for high-throughput applications.
How does the kit ensure endotoxin-free DNA?
The RevDNA™ Mega Plasmid Kits include an integrated endotoxin removal step, ensuring that the purified DNA is free from endotoxins (<0.1 EU/µg DNA), critical for sensitive applications such as transfection.
Can the RevDNA™ Mega Plasmid Kits be used for transfection of primary cells?
Yes, the purified DNA obtained using these kits is suitable for the transfection of primary cells, providing reproducible and reliable results, even in sensitive cell types.
Is the purification process with the RevDNA™ Mega Plasmid Kits compatible with automated systems?
Yes, these kits can be integrated into automated workflows, offering flexibility and scalability for high-throughput laboratories.
| Reactions | Giga Kit: 5 reactions, Mega Kit: 5 reactions |
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