The Avam® Real Merase™ qPCR system is cutting-edge technology targeting high-precision quantitative PCR applications. Combining the best technology with user-friendly features ensures world-class performance and reliability for the modern laboratory. Whether it’s research in genomics, diagnostics, or molecular biology, the Real Merase™ qPCR system offers you the tools to arrive at correct results reproducibly.
Real Merase™ qPCR System
Introduction
The Avam® Real Merase™ qPCR system is an advanced, high-precision instrument designed for quantitative PCR applications. Built to meet the rigorous demands of modern laboratories, this system combines cutting-edge technology with user-friendly features to deliver reliable, reproducible results across genomics, diagnostics, and molecular biology research.
Design and User Experience
The Real Merase™ qPCR system features a sleek, modern design that fits seamlessly into any laboratory setting. With its 10.1-inch HD touchscreen, researchers can easily navigate complex protocols and monitor experiments in real-time. The system supports both standalone operation and PC control (Windows 8 and higher), offering flexibility to meet diverse laboratory workflows. The automatic sample tray enables integration with automated workstations and plate handlers for unmanned detection, enhancing lab efficiency.
Advanced Optical System
The Real Merase™ is equipped with a full-spectrum, high-power LED excitation light source and a highly sensitive Photomultiplier Tube (PMT) detector. This advanced optical system ensures precise and accurate detection of fluorescence signals, with minimal cross-talk between the six fluorescence channels. The system’s top-scanning detection provides high sensitivity, ideal for detecting low-abundance targets in complex assays.
Fluorescence channels (6 total):
- Channel 1: FAM/SYBR Green I
- Channel 2: JOE/HEX/TET/IC
- Channel 3: NED/TAMRA/Cy3
- Channel 4: ROX/Texas Red
- Channel 5: Cy5
- Channel 6: Cy5.5
Excitation wavelength range: 400–800 nm
Detection wavelength range: 500–800 nm
Thermal Cycling Technology
The Real Merase™ qPCR system is powered by Peltier Module technology, providing precise and consistent temperature control for superior thermal cycling performance. The Peltier module allows for rapid heating and cooling with a maximum rate of ≥6°C/s, ensuring faster cycle times and improved experiment efficiency.
- Block temperature range: 4°C to 105°C
- Block temperature accuracy, precision, and uniformity: ≤±0.1°C, ensuring uniform thermal conditions across all wells.
- Gradient temperature range: 30°C to 100°C with a 1–40°C temperature difference for assay optimization.
- Two temperature control modes: Block mode and tube mode, offering flexibility depending on your specific experiment requirements.
Throughput and Consumables
The Real Merase™ supports 96-well plate formats, allowing for high-throughput experiments. It is compatible with a wide range of consumables, making it suitable for various experimental setups:
- PCR single tubes and strips (0.2 mL and 0.1 mL)
- 96-well half-skirted and no-skirted plates
With a dynamic range of 1–10^10 copies, the system is capable of detecting both high- and low-abundance targets, ensuring high flexibility in research applications.
Sample Volume and Heated Lid
The system accommodates sample volumes ranging from 5 to 100 µL, providing flexibility for different experimental needs. The heated lid operates within a temperature range of 30°C to 110°C (default at 105°C), ensuring even heating and preventing condensation, which is crucial for maintaining sample integrity.
Connectivity and Data Management
The Real Merase™ qPCR system offers robust connectivity with:
- Two USB Type-A ports
- One USB Type-B port
- RJ-45 Ethernet port
These options allow for seamless data transfer, integration with external devices, and communication with laboratory data management systems. The power-off protection feature ensures that experimental data is automatically recovered after any power interruptions, preventing data loss.
Software Features
The Real Merase™ is equipped with an intuitive, user-friendly software suite that makes running qPCR experiments fast and easy. Unlike outdated systems that require relearning, Avam’s software is designed so that users can set up and run experiments within minutes, eliminating unnecessary complexity.
- Remote Monitoring and Control: The system supports remote monitoring and control, allowing users to track the progress of their experiments and manage multiple systems from a single interface.
- Absolute and relative quantification
- Melting curve analysis
- SNP genotyping
- High-resolution melting (HRM)
- Quick-run modes
These features offer a wide range of qPCR capabilities, from routine assays to more complex diagnostic applications.
Repeatability and Scan Modes
The Real Merase™ qPCR system ensures high repeatability and consistency between runs. It offers flexible scan modes, including full-plate scans and line scans, allowing researchers to tailor the detection process to meet specific experimental needs. The fluorescence stability (CV ≤3%) ensures reliable and reproducible results, even in high-throughput applications.
Conclusion
The Avam® Real Merase™ qPCR system is a next-generation platform designed to simplify and enhance molecular biology and diagnostics research. With Peltier Module technology for precise thermal cycling, an advanced optical system, and a highly intuitive software interface, the Real Merase™ ensures accurate, reliable, and reproducible results. Whether for basic research or clinical applications, the Real Merase™ is an essential tool for achieving high-quality, efficient qPCR workflows.
Additional Products
| Instrument Color | Carbon Aluminum, Cloud Aluminum |
|---|
Updating…