ProteoGrade™ Nuclear Protein Kit

$680.00

Efficiently isolate high-quality nuclear proteins from mammalian cell cultures for a wide range of molecular biology applications.

Purchase this item and get 68 Points
Purchase this item and get 68 Points
Product Description

The ProteoGrade™ Nuclear Protein Kit represents a paradigm shift in the field of molecular biology by offering researchers a cutting-edge tool for isolating nuclear proteins with unparalleled efficiency and precision. Engineered with meticulous attention to detail, this kit employs a streamlined protocol that ensures the reproducible extraction of high-purity nuclear proteins from cultured mammalian cells.

Designed to meet the exacting demands of modern research, the ProteoGrade™™™ Nuclear Protein Kit offers a comprehensive solution for researchers seeking to unravel the complexities of nuclear protein biology. By providing a standardized and reproducible fractionation process, this kit enables scientists to obtain nuclear protein fractions virtually devoid of cytosolic contaminants, thereby greatly reducing sample complexity and facilitating downstream analyses.

The principle underlying the ProteoGrade™ Nuclear Protein Kit is rooted in its innovative approach to nuclear protein extraction. Through a series of carefully orchestrated steps involving cell lysis, centrifugation, and selective extraction, the kit enables the efficient separation of nuclear proteins from other cellular components. By leveraging high-salt buffers to dissociate nuclear binding proteins from DNA and RNA, the kit ensures the isolation of functionally active nuclear proteins in a highly purified form.

Researchers can rely on the ProteoGrade™ Nuclear Protein Kit to deliver consistently high-quality nuclear protein fractions, making it an indispensable tool for a wide range of applications, including SDS-PAGE analysis, mass spectrometry, activity assays, and protein-protein interaction studies. Whether investigating the role of nuclear proteins in cellular processes, exploring signaling pathways, or identifying potential therapeutic targets, this kit empowers researchers to delve deeper into the intricacies of nuclear biology with confidence and precision.

In summary, the ProteoGrade™ Nuclear Protein Kit represents a groundbreaking advancement in the field of molecular biology, offering researchers a versatile and reliable solution for isolating nuclear proteins from mammalian cell cultures with unparalleled efficiency, reproducibility, and purity. With its user-friendly format and robust performance, this kit is poised to revolutionize the way researchers study nuclear protein function and regulation, paving the way for new discoveries and insights into the complex mechanisms governing cellular behavior.

Kit Contents

● Buffers and reagents for efficient protein extraction
● Protease Inhibitor Solution to maintain protein integrity
● Benzonase for nucleic acid removal

Technical Parameters

● Applications: SDS-PAGE, mass spectrometry
● Fractions isolated: Three fractions
● Species: Mammal
● Sample size: Up to 5 x 10^6 cells
● Binding capacity/yield: 200–300 µg
● Start material: Cell cultures

Applications

● SDS-PAGE analysis
● Mass spectrometry
● Activity assays
● Protein-protein interaction studies

Product FAQs
What is the sample size requirement for using the ProteoGrade ™ Nuclear Protein Kit?

The kit is suitable for processing samples of up to 5 x 10^6 cells.

Can the isolated nuclear proteins be used for mass spectrometry analysis?

Yes, the kit provides nuclear protein fractions suitable for mass spectrometry analysis.

Does the kit include Protease inhibitors to maintain protein integrity during extraction?

Yes, the kit contains Protease Inhibitor Solution to preserve protein integrity.

Is the ProteoGrade™ Nuclear Protein Kit compatible with samples from other species besides mammals?

The kit is specifically designed for use with mammalian cell cultures.

Preparation Size

12 preps

SKU: WT4310 Category:
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