Obtain high-purity mitochondrial preparations with ease using the ProteoGrade™ Mitochondria Isolation Kit, designed for molecular biology applications.
ProteoGrade™ Mitochondria Isolation Kit
$540.00
Purchase this item and get Product Description
The ProteoGrade™ Mitochondria Isolation Kit revolutionizes the process of mitochondrial isolation by offering a streamlined, standardized procedure that guarantees high-purity mitochondrial preparations with intact biological activity. Unlike traditional methods, which are laborious and time-consuming, this kit simplifies the process, making it accessible to researchers without access to specialized equipment like ultracentrifuges.
Through innovative chemistry and a carefully designed protocol, this kit ensures the efficient separation of mitochondrial and cytosolic fractions, minimizing cross-contamination and yielding highly enriched mitochondrial fractions. This purity is crucial for downstream applications, where contamination could interfere with the accuracy of results.
Moreover, the isolated mitochondria retain their full biological activity and native conformation, making them ideal for a wide range of studies. Whether investigating apoptosis, conducting proteomic analyses, or exploring drug discovery, researchers can rely on the integrity and purity of the mitochondrial preparations obtained using this kit.
By offering convenience, reproducibility, and exceptional performance, the ProteoGrade™ Mitochondria Isolation Kit empowers researchers to delve deeper into mitochondrial biology with confidence, unlocking new insights into cellular function and disease mechanisms.
Kit Contents
Buffers and reagents for mitochondrial isolation
Technical Parameters
● Applications: SDS-PAGE, mass spectrometry, activity assays
● Sample size: 5 x 10^6 – 2 x 10^7 cells, 20 mg – 60mg
● Binding capacity/yield: 20–80 μg
● Species: Eukaryotes
● Fractions isolated: One fraction
● Start material: Cells, Tissue
Applications
● SDS-PAGE: Protein separation and analysis.
● Mass Spectrometry: Identification and quantification of mitochondrial proteins.
● Activity Assays: Assessment of mitochondrial function.
Product FAQs
What type of cells or tissues can be used as starting material for mitochondrial isolation with this kit?
The kit is compatible with various cell types and tissues from eukaryotic organisms.
Can this kit be used for the isolation of specific types of glycoproteins from mitochondria?
The kit does not specifically target glycoproteins; however, it can isolate mitochondria containing glycoproteins for subsequent analysis.
How does the performance of this kit compare to traditional methods of mitochondrial isolation?
The kit offers faster, more reproducible, and higher-purity mitochondrial preparations compared to traditional methods, without the need for an ultracentrifuge.
What downstream applications are suitable for the isolated mitochondria?
The isolated mitochondria are suitable for a wide range of applications, including SDS-PAGE, mass spectrometry, activity assays, and studies related to apoptosis, proteomics, and drug discovery.
| Preparation Size | 12 preps |
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