ProteoGrade™ FFPE Tissue Kit

The ProteoGrade™ FFPE Tissue Kit revolutionizes protein extraction from formalin-fixed, paraffin-embedded (FFPE) tissue samples, unlocking a treasure trove of archived specimens for proteomic analysis. Traditional FFPE samples, once limited to histological studies, can now be interrogated at the protein level with unparalleled depth and detail.

Utilizing proprietary chemistry, this innovative kit reverses the crosslinking induced by formalin fixation, liberating full-length proteins from the entangled network of molecular bonds. The result is a standardized and quantitative extraction process that yields proteins suitable for a myriad of downstream applications, including western blotting, protein arrays, and mass spectrometry.

With a streamlined protocol, the ProteoGrade™ FFPE Tissue Kit offers efficiency and reliability, ensuring reproducible protein extraction from FFPE tissue sections. The protein yields obtained are comparable to those from frozen material, enabling direct comparison between different tissues and facilitating the identification of disease-specific markers.

The principle behind the kit lies in its ability to dismantle the chemical bonds formed during formalin fixation, thereby restoring the native structure and functionality of proteins within FFPE samples. This breakthrough technology empowers researchers to explore protein expression profiles in archived FFPE tissues, providing invaluable insights into disease mechanisms and biomarker discovery.

The procedure is straightforward: FFPE tissue sections undergo deparaffinization followed by incubation in the Extraction Buffer. After centrifugation, the extracted proteins are recovered in the supernatant, ready for analysis. Whether loading onto an SDS-PAGE gel or into an automated immunoassay system such as Simple Western™, no specialized equipment or additional processing steps are required.

With its standardized approach and robust performance, this kit empowers researchers to unlock the proteomic potential of FFPE tissue archives and advance our understanding of complex diseases.