ParaPrep™ DNA Extraction Kits

ParaPrep™ DNA Extraction Kits

Price range: $227.00 through $805.00

Extract high-yield, artifact-free DNA from challenging FFPE tissue samples with the ParaPrep™ DNA Extraction Kits, featuring automated protocols and advanced uracil-N-glycosylase (UNG) technology for enhanced accuracy.

Purchase this item and get 22-80 Points
Purchase this item and get 22-80 Points
Product Description

The ParaPrep™ DNA Extraction Kits offer a comprehensive solution for purifying amplifiable DNA from challenging formalin-fixed, paraffin-embedded (FFPE) tissue samples. Utilizing a sophisticated two-step lysis process, this kit ensures high DNA extraction efficiency even from hard-to-lyse samples, maximizing DNA yields from limited inputs.

One notable feature of this kit is its innovative paraffin removal solution, which facilitates efficient deparaffinization without the need for xylene or similar solvents. This wash-free deparaffinization method minimizes the risk of sample loss, which can occur with traditional solvent-based deparaffinization techniques involving multiple wash steps.

Additionally, the ParaPrep™ DNA Extraction Kits offer an optional uracil-N-glycosylase (UNG) step, designed to remove deaminated cytosine artifacts. By reducing the incidence of C→T/G A transitions, commonly observed in FFPE samples due to cytosine deamination, this UNG treatment helps mitigate the risk of nucleotide read errors, particularly crucial for downstream applications such as next-generation sequencing (NGS).

Overall, the ParaPrep™ DNA Extraction Kits delivers high-quality, amplifiable DNA suitable for a wide range of molecular biology applications, including PCR, digital PCR (dPCR), and NGS. With optimized protocols, reliable performance, and the ability to process FFPE samples with ease, this kit represents a valuable tool for researchers working with challenging FFPE tissues.

Kit Contents

● ParaPrep™ DNA FFPE Card
● Disposable filter-tips and tip-holders
● Tubes
● Paraffin Removal Solution
● Avam® Proteinase K
● Avam® RNase A
● Nuclease-free water
● Buffers
● Avam® Uracil-N-Glycosylase can be purchased separately or in a kit.

Technical Parameters​

● Applications: PCR, digital PCR, next-generation sequencing
● Elution volume: 60 and 100 µl
● Format: Magnetic bead
● Main sample type: Formalin-fixed paraffin-embedded tissue samples
● Technology: Silica technology
● Sample amount: Tissue sections, each with a thickness of 5–10 µm,
for a total volume of 4 mm3

Applications

● PCR amplification
● Digital PCR
● Next-generation sequencing

Product FAQs
Can this kit be used for RNA extraction?

No, this kit is specifically designed for DNA extraction from FFPE tissue samples.

How long can the extracted DNA be stored?

The DNA can be stored at -30 to -15°C for long-term preservation. However, it is recommended to aliquot and store the DNA in suitable storage conditions to prevent degradation.

Does the kit include all necessary reagents for the entire DNA extraction process?

Yes, the ParaPrep™ DNA Extraction Kits come with all essential reagents required for the complete DNA extraction process, including paraffin removal solution, proteinase K, RNase A, and buffers.

Can this kit be used with samples other than FFPE tissues?

The kit is specifically optimized for DNA extraction from formalin-fixed, paraffin-embedded tissue samples. While it may be possible to adapt the protocol for other sample types, optimal results are achieved with FFPE tissues.

Preparation Size

DNA FFPE Kit: 48, DNA FFPE UNG Kit: 48, Uracil-N-Glycosylase: 50
SKU: PE9023 Category:
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